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Hyung-Woo Kwak, Kook-Young Kim, Ji-Sang Han, Seung-Young Yu; Changes Of Retinal Thickness In Diabetic Rats (OLETF). Invest. Ophthalmol. Vis. Sci. 2012;53(14):5485.
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To compare thickness changes in retina of Otsuka Long-Evans Tokushima fatty (OLETF) rats, a model of type 2 diabetes and Long-Evans Tokushima Otsuka (LETO) rats using spectral domain optical coherence tomography (SD-OCT).
To evaluate reliability, we performed repeated measurements under controlled condition, and confirmed repeatability. 22 eyes of 11 OLETF rats and 38 eyes of 19 LETO rats were scanned using spectral domain optical coherence tomography (Spectralis HRA+ OCT, Heidelberg engineering, Heidelberg, Germany) at the ages of 12 weeks and 36 weeks (20 weeks after the onset of diabetes). Retinal thickness and retinal nerve fiber layer (RNFL) thickness were measured 1300 µm from the ONH center.
The coefficients of variation (COV) and intraclass coefficients (ICC) were 1.8%, 0.941 for total retinal thickness and 5.2%, 0.764 for RNFL thickness. The total retinal thickness of OLETF rats at the age of 36weeks (235.98±5.42 µm) significantly decreased (p=0.042) compared with that of LETO rats (240.58±6.99 µm). The RNFL thickness of OLETF rats (22.07±1.94µm) significantly decreased (p=0.000) compared with that of LETO rats (24.37±2.02µm). Inner nuclear layer, outer nuclear layer, and outer plexiform layer demonstrated no significant difference in the thickness between two groups.
The total retinal thickness and the RNFL thickness in type 2 diabetes model OLETF rats significantly decreased. This demonstrated the possibility of OLETF rats for animal model of neurodegeneration in the retina. We also used non-invasive SD-OCT scanning instead of histologic examination to analyze the structure and morphology of retina, offering possibility of longitudinal study for diabetic retinopathy with animal model.
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