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Francine F. Behar-Cohen, Elodie Touchard, Berdugo Marianne, Michèle Savoldelli, Marie-Christine Naud, Jean-Claude Jeanny; A Novel Method To Transfect Retinal Pigment Epithelial Cells Without Detaching The Retina. Invest. Ophthalmol. Vis. Sci. 2012;53(14):5580.
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Sub retinal route of viral vectors is the validated method to transfect retinal cells at the site of retinal detachment. A safer method would benefit to macular targeting. We have evaluated a novel method that combines the administration of plasmid DNA in the suprachoroidal space of rat eyes with scleral electroporation procedure.
Optimal injection, current electroporation parameters and electrode geometry were evaluated using a plasmid encoding Lac-Z. Safety of the procedure was monitored using fluorescein angiography, electroretinography and histology.
Suprachoroidal injection induces a large area of diffusion. Scleral electroporation using external electrodes efficiently transfects not only choroidal cells but also retinal pigment epithelial cells and photoreceptors using a CMV promoter. No histologic or functional resulted from this minimally invasive procedure
We describe here a novel minimally invasive, non-viral gene therapy method for the transfection of RPE cells without any retinal detachment. Further analysis will be performed to optimize cell specific targeting and long-term expression.
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