March 2012
Volume 53, Issue 14
Free
ARVO Annual Meeting Abstract  |   March 2012
Temporal Interactions Between the b-wave and d-wave of the Human Electroretinogram
Author Affiliations & Notes
  • pan Shi
    Psychological and Brain Sciences, University of Louisville, Louisville, Kentucky
  • Kate A. Godwin
    Psychological and Brain Sciences, University of Louisville, Louisville, Kentucky
  • Paul J. DeMarco
    Psychological and Brain Sciences, University of Louisville, Louisville, Kentucky
  • Footnotes
    Commercial Relationships  pan Shi, None; Kate A. Godwin, None; Paul J. DeMarco, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science March 2012, Vol.53, 5709. doi:
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      pan Shi, Kate A. Godwin, Paul J. DeMarco; Temporal Interactions Between the b-wave and d-wave of the Human Electroretinogram. Invest. Ophthalmol. Vis. Sci. 2012;53(14):5709.

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Abstract

Purpose: : We examined the temporal interactions between the b-wave and d-wave of the human electroretinogram (ERG) and the influence of each on the amplitude of the ERG response.

Methods: : Data were collected from nine human subjects using increment and decrement flashes and standard ERG recording techniques. For increment flashes, intensities from 75 to 900 cd/m2 were presented on a 25 cd/m2 background using stimulus durations of 5 ms, 20 ms and 75 ms. For these conditions the amplitude of the b-wave was measured. For decrement flashes, flash intensities from -100 to -300 cd/m2 were presented on a 325 cd/m2 background with stimulus durations of 5 ms, 20 ms and 75 ms. For these conditions the amplitude of the d-wave was measured. The data were analyzed using a 2-factor repeated measures ANOVA.

Results: : For increment flashes, the amplitude of the b-wave increased as a function of flash intensity for all flash durations and showed a plateau at higher intensities, but the slope and morphology of the intensity-response functions differed for each flash duration. There was a statistical main effect for amplitude across duration, revealing that the b-wave amplitude decreased as duration increased. For decrement flashes, the slope of the d-wave intensity-response function was similar across flash durations and did not show a plateau. However, the d-wave amplitude was significantly lower for 5 ms duration flashes compared to both 20 and 75 ms duration flashes, which did not differ from one another. As flash duration is increased, the b-wave and d-wave responses become separate entities and the interaction between the two changes.

Conclusions: : For short duration flashes, the b-wave and d-wave interact to influence the ERG response. For increment flashes, the amplitude of the b-wave is increased by the d-wave response to a brief flash, but for decrement flashes the d-wave is decreased by the b-wave response to a brief flash. The flash duration at which the two responses become independent differs, suggesting a temporally dependent interaction between the generators of the two waveforms. These results suggest that the standard, brief-flash, increment and decrement ERG is a result of both ON- and OFF-pathway activity.

Keywords: electroretinography: non-clinical 
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