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Stefan Mergler, Monika Valtink, Monika Sahlmüller, Peter S. Reinach, Katrin Engelmann, Uwe Pleyer; Transient Receptor Potential Melastatin 8 (TRPM8) Channels Mediate Complex Calcium Responses in Human Corneal Endothelial Cells. Invest. Ophthalmol. Vis. Sci. 2012;53(14):6015.
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© ARVO (1962-2015); The Authors (2016-present)
The cold receptor TRPM8 is a member of the melastatin-type transient receptor potential ion channel family. Activation by cold or by cooling agents (menthol, icilin) induces a transient rise in intracellular free calcium concentration ([Ca2+]i). Our previous studies demonstrated that Ca2+-permeable heat-sensitive TRP vanilloid channels (TRPV1-4) play a role in temperature-sensing in human corneal endothelial cells (HCEC) (Exp Eye Res 2010, 90, 758-770; 2011, 93, 710-719). Here, we extend our earlier study by probing for functional TRPM8 expression in HCEC.
Intracellular free Ca2+ ([Ca2+]i) was measured in fura2-loaded human corneal endothelial cells (HCEC-12 cell line). TRP channel currents were measured by the planar patch-clamp technique. Nonselective cation currents were analyzed by a color-coded plot method.
This study demonstrates for the first time functional expression of TRPM8 in HCEC cells and its complex role in regulating [Ca2+]i as well as cold-sensing. In addition to thermosensitive TRPV1-4, TRPM8 reveals functional activity in Ca2+ regulation and contributes to HCEC homeostasis. Its potential role in the pathophysiology of HCEC such as apoptosis and cell loss has still to be determined.
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