March 2012
Volume 53, Issue 14
Free
ARVO Annual Meeting Abstract  |   March 2012
Development of a Drug released Soft Contact Lens that Releases Antibiotics in a Sustained Manner
Author Affiliations & Notes
  • Shinichiro Kobayakawa
    1st Dept of Ophthalmology, Toho University, Tokyo, Japan
  • Toru Matsunaga
    SEED Co Ltd, Kounosu-shi, Japan
  • Kohji Kakisu
    1st Dept of Ophthalmology, Toho University, Tokyo, Japan
  • Yoshiko Yamazaki
    SEED Co Ltd, Kounosu-shi, Japan
  • Takao Sato
    SEED Co Ltd, Kounosu-shi, Japan
  • Tetsuo Tochikubo
    1st Dept of Ophthalmology, Toho University, Tokyo, Japan
  • Footnotes
    Commercial Relationships  Shinichiro Kobayakawa, None; Toru Matsunaga, SEED Co Ltd (E); Kohji Kakisu, None; Yoshiko Yamazaki, SEED Co Ltd (E); Takao Sato, SEED Co Ltd (E); Tetsuo Tochikubo, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science March 2012, Vol.53, 6102. doi:
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      Shinichiro Kobayakawa, Toru Matsunaga, Kohji Kakisu, Yoshiko Yamazaki, Takao Sato, Tetsuo Tochikubo; Development of a Drug released Soft Contact Lens that Releases Antibiotics in a Sustained Manner. Invest. Ophthalmol. Vis. Sci. 2012;53(14):6102.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : We developed a new soft contact lens (SCL) of a hydrogel material capable of releasing antibiotics in a sustained manner. The purpose of this study was to investigate the penetration of the antibiotics to the eye by the drug released SCL (DR-SCL) in a rabbit model.

Methods: : Gatifloxacin (GFLX) 0.3% and moxifloxacin (MFLX) 0.5% eye drops were used. In twenty-seven rabbits, the antibiotics concentration of cornea, aqueous humor and crystalline lens was measured with high-performance liquid chromatography (HPLC). Those samples were collected at 10, 30, and 60 minutes after three times topical administration at 15-min intervals by those eye drops (eye drops group), and through the application with DR-SCL (DR-SCL group), also collected at 24, 48, and 72 hours through the application with DR-SCL.

Results: : The maximum GFLX concentration in cornea were 0.7μg/ml (@10 min), and the MFLX concentration were 2.4μg/ml (@10 min) in eye drops group. Whereas, those were 9.5μg/ml (@60 min) and 23μg/ml (@60 min) in DR-SCL group, respectively. Those concentrations were significant differences between eye drops group and DR-SCL groups at 10 min. The maximum GFLX concentrations in aqueous humor were 1.1μg/ml (@60 min), and the MFLX concentration were 6.4μg/ml (@60 min) in eye drops group. Whereas, those were 13.4μg/ml (@60 min) and 60μg/ml (@60 min) in DR-SCL group, respectively. Those concentrations were significant differences between eye drops group and DR-SCL groups at 60 min. The maximum GFLX concentrations in crystalline lens were 0.02μg/ml (@60 min), and the MFLX concentration were 0.17μg/ml (@30 min) in eye drops group. Whereas, those were 0.05μg/ml (@60 min) and 0.3μg/ml (@60 min) in DR-SCL group, respectively. Those concentrations in crystalline lens were not significant differences between eye drops group and DR-SCL groups. Those concentrations in cornea for GFLX in DR-SCL group decreased from 0.69μg/ml to 0.04μg/ml, and for MFLX decreased from 4.22μg/ml to 0.89μg/ml through 72 hours. Those concentrations in aqueous humor for GFLX decreased from 2.6μg/ml to 0.08μg/ml, and for MFLX decreased from 7.9μg/ml to 3.2μg/ml. Those concentrations in crystalline lens for GFLX decreased from 0.3μg/ml to 0.08μg/ml, and for MFLX decreased from 0.6μg/ml to 0.3μg/ml.

Conclusions: : Antibiotics concentrations of DR-SCL group either in aqueous humor or in cornea were approximately ten times higher than those of eye drops group. Those to crystalline lens in DR-SCL group were similar or higher than those in eye drops group. Moreover, DR-SCL sustained release of antibiotics through 72 hours. DR-SCL may be an expectable drug delivery system into the interior of the eye.

Keywords: contact lens • aqueous • crystallins 
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