March 2012
Volume 53, Issue 14
Free
ARVO Annual Meeting Abstract  |   March 2012
Vasoactive Intestinal Peptide Modulates Growth Factors and their Receptors in Normal and Infected Cornea
Author Affiliations & Notes
  • Sharon A. McClellan
    Anatomy & Cell Biology, Wayne State Univ School of Med, Detroit, Michigan
  • Xiaoyu Jiang
    Anatomy & Cell Biology, Wayne State Univ School of Med, Detroit, Michigan
  • Ronald P. Barrett
    Anatomy & Cell Biology, Wayne State Univ School of Med, Detroit, Michigan
  • Yunfan Zhang
    Anatomy & Cell Biology, Wayne State Univ School of Med, Detroit, Michigan
  • Megan E. Foldenauer
    Anatomy & Cell Biology, Wayne State Univ School of Med, Detroit, Michigan
  • Linda D. Hazlett
    Anatomy & Cell Biology, Wayne State Univ School of Med, Detroit, Michigan
  • Footnotes
    Commercial Relationships  Sharon A. McClellan, None; Xiaoyu Jiang, None; Ronald P. Barrett, None; Yunfan Zhang, None; Megan E. Foldenauer, None; Linda D. Hazlett, None
  • Footnotes
    Support  NEI R01 EY02986 and P30 EY04068
Investigative Ophthalmology & Visual Science March 2012, Vol.53, 6137. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Sharon A. McClellan, Xiaoyu Jiang, Ronald P. Barrett, Yunfan Zhang, Megan E. Foldenauer, Linda D. Hazlett; Vasoactive Intestinal Peptide Modulates Growth Factors and their Receptors in Normal and Infected Cornea. Invest. Ophthalmol. Vis. Sci. 2012;53(14):6137.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: : Vasoactive intestinal peptide (VIP), an anti-inflammatory neuropeptide, down-regulates pro-inflammatory cytokines, and upregulates growth factors (GF), promoting healing in P. aeruginosa keratitis. Whether VIP is required for production of GF or their receptors in the cornea before and after infection is untested and the purpose of this study.

Methods: : VIP-/- vs wild type C57BL/6 mice were infected and disease graded and recorded by slit lamp photography. PCR array was used to test fold differences in a panel (84 genes) of GF and their receptors in these mice. RT-PCR was used to confirm results for selected genes from the array. Immunostaining (IHC) was used to localize several GF and their receptors in the normal and infected eyes of C57BL/6 wild type and VIP-/- mice.

Results: : Infection in VIP -/- mice resulted in earlier [2 days postinfection (p.i.)] corneal perforation when compared to wild type mice in which perforation occurs at 5-7 days p.i. Thus, array and RT-PCR experiments were done in normal cornea and at 2 days p.i. In VIP-/- mice, GF receptors for EGF and HGF were significantly downregulated at 2 days p.i.; FGF receptor was similar between groups. GF, EGF and HGF were upregulated in VIP-/- while FGF was downregulated at 2 days p.i. IHC for GF and their receptors in the normal cornea of VIP-/- vs wild type mice, revealed less EGF, similar HGF, and more FGF epithelial staining. For GF receptors, more EGF and FGF, and less HGF epithelial staining was seen. At 1 day p.i., VIP-/- mice had more intense EGF, less HGF, and similar FGF staining vs wild type mice. For GF receptor staining, VIP-/- mice showed less intense EGF, HGF and FGF staining vs wild type mice.

Conclusions: : The data reiterate the importance of VIP for healing in the infected cornea and provide new evidence that VIP is required for optimum GF and GF receptor production, particularly after infection.

Keywords: bacterial disease • neuropeptides • growth factors/growth factor receptors 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×