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Andrea Leonardi, Diego Faggian, Alvise La Gloria Valerio, Federico Piliego, Laura Motterle, Mario Plebani; Microarray Based Ige Detection In Tears Of Vernal Keratoconjunctivitis Patients. Invest. Ophthalmol. Vis. Sci. 2012;53(14):6186.
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© ARVO (1962-2015); The Authors (2016-present)
To evaluate whether a new commercial micoarray test for the measurement of specific immunoglobulin (Ig) E, could be used for the diagnosis of allergic sensitization in vernal keratoconjunctivitis (VKC).
Serum and tears samples were collected from 10 active VKC patients (3 females, 7 males) and 10 age-matched normal subjects. Skin prick test, symptoms score and full ophthalmological examination were performed. Specific serum and tear IgE were assayed using ImmunoCAP ISAC, a microarray containing 103 components derived from 47 allergens.
Normal subjects resulted negative for the presence of specific IgE both in serum and in tears. Of the 10 VKC patients, 6 resulted positive to specific IgE. In 3 of these 6 patients, specific IgE were found positive only in tears. Cross reactivity between specific markers were found in 3 patients. Grass, tree, mites, animal but also food allergen specific IgE were found in tears. Conjunctival provocation test performed out of season confirmed the specific local conjunctival reactivity.
Multiple specific IgE measurements with single protein allergens using a microarray technique in tear samples is a useful, simple and non-invasive diagnostic tool. ImmunoCAP ISAC detects allergen sensitization at component level and adds important information by defining both cross and co-sensitization to a large variety of allergen molecules. The presence of specific IgE only in tears of VKC patients reinforce the concept of possible local sensitization.
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