March 2012
Volume 53, Issue 14
Free
ARVO Annual Meeting Abstract  |   March 2012
Up-regulation Of Soluble Amyloid Beta And Down-regulation Of Soluble RAGE In The Vitreous Of Age-related Macular Degeneration Patients
Author Affiliations & Notes
  • Frances Fan
    Ophthalmology, Hematology and Oncology,
    Georgia Health Sciences University, Augusta, Georgia
  • Anna Lisa Montemari
    Experimental Medicine and Pathology, University of Rome La Sapienza, Rome, Italy
  • Stefania Rossi
    Istituto Superiore Di Sanita, Rome, Italy
  • Giovanni Parisi
    Fondazione GB Bietti, Rome, Italy
  • Folami Lamoke
    Pharmacology and Toxicology,
    Georgia Health Sciences University, Augusta, Georgia
  • Francesco Facchiano
    Ophthalmology, Hematology and Oncology,
    Istituto Superiore Di Sanita, Rome, Italy
  • Guido Ripandelli
    Fondazione GB Bietti, Rome, Italy
  • Manuela Bartoli
    Ophthalmology, Hematology and Oncology,
    Georgia Health Sciences University, Augusta, Georgia
  • Footnotes
    Commercial Relationships  Frances Fan, None; Anna Lisa Montemari, None; Stefania Rossi, None; Giovanni Parisi, None; Folami Lamoke, None; Francesco Facchiano, None; Guido Ripandelli, None; Manuela Bartoli, None
  • Footnotes
    Support  Fondazione G.B. Bietti and Italian Ministry of Health
Investigative Ophthalmology & Visual Science March 2012, Vol.53, 6423. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Frances Fan, Anna Lisa Montemari, Stefania Rossi, Giovanni Parisi, Folami Lamoke, Francesco Facchiano, Guido Ripandelli, Manuela Bartoli; Up-regulation Of Soluble Amyloid Beta And Down-regulation Of Soluble RAGE In The Vitreous Of Age-related Macular Degeneration Patients. Invest. Ophthalmol. Vis. Sci. 2012;53(14):6423.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: : The identification of aggregates of amyloid beta (A-beta) peptides in drusen has implicated these neurotoxic factors in the pathogenesis of age-related macular degeneration (ARMD). To further determine whether production of A-beta could be involved in the pathogenesis of ARMD, we have conducted proteomic studies assessing the presence of soluble amyloid beta (sA-beta) peptides and the soluble isoform of its receptor (sRAGE) as these are considered markers of disease progression in A-beta-mediated neurodegenerative diseases including Alzheimer’s disease (AD).

Methods: : Western blotting, dot blot and mass spectrometry were used to assess sA-beta and sRAGE in undiluted vitreous of ARMD patients (n=12) or control patients (n=34) undergoing vitrectomy for unrelated pathologies (trauma, macular pucker and others).

Results: : Soluble A-beta was detected in the vitreous of about 30% of the patients affected by ARMD and only in 5% of the control patients. Soluble RAGE was detected in all the analyzed patients, however the ARMD patients showed a significant decrease in sRAGE levels (p<0.003) in the undiluted vitreous.

Conclusions: : Our data show that soluble A-beta is found in the vitreous of patients affected by ARMD whereas the soluble isoform of A-beta receptor, the sRAGE, is significantly decreased. These data are in agreement with similar findings obtained in Alzheimeir’s patients, where the up-regulation of sA-beta associated with decreased levels of the sRAGE, involved in its clearance, are markers of disease progression. Thus, the obtained results further support a pathogenic role for A-beta in ARMD.

Keywords: age-related macular degeneration • vitreous • proteomics 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×