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Violeta Sanchez-vallejo, Miguel Flores-Bellver, Raquel Álvarez-Nölting, Siv Johnsen-Soriano, María Miranda, Francisco Javier Romero Gómez; Changes In The Expression Of Genes Related To Oxidative Stress In Rd1 Mice.. Invest. Ophthalmol. Vis. Sci. 2012;53(14):6559.
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© ARVO (1962-2015); The Authors (2016-present)
Retinitis Pigmentosa (RP) is a neurodegenerative disease that affects photoreceptors and causes blindness in humans. In previous studies we have shown that oxidative stress plays an important role in retinal degeneration. The purpose of this study was to investigate the changes in the expression of various genes related to oxidative stress and antioxidant defense in the rd1 retina at post-natal day (PN) 11. The rd1 mouse has a mutation in the exon 7 of the subunit beta of the rod PDE6 gene that results in a non functional PDE6 enzyme, leading to an accumulation of cGMP in rod photoreceptors. The degenerative process in this model starts very early, around PN 11. The peak of rod photoreceptor cell death occurs between PN11-14.
Animals were treated in accordance to the ARVO statement for the use of animals in ophthalmic and vision research. We obtained retina from wild type and rd1 mice at day PN11. Retinas were homogenized in prechilled 0.2 M potassium phosphate buffer, pH 7.0. This homogenate was used to determinate the expression of 84 genes related to oxidative stress by quantitative PCR (PAMM-065F, SABiosciences).
The expression of different genes that are related to oxidative stress and antioxidant defense were significantly down-regulated: Mpp4, Slc38a1, Mpo, Tmod1, Ehd2, Ift172, Srxn1, Ercc2, Prnp, Gab1, Aqr, Prdx6-ps1, Actb, and others significantly up-regulated: Xirp1, Gpx5, Aass, Nqo1, Ccs, Mb, Txnip, Glutathione peroxidase 8, Prdx4, Prdx3, Sod1, Hprt, Gpx1 in retinas of rd1 mice in comparation with wild type mice.
We have found an overall disequilibrium in genes related to oxidative stress and antioxidant defense. Further studies are needed to confirm the importance of each disregulation in oxidative stress-related genes in Retinitis Pigmentosa and other retinal degenerations.
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