March 2012
Volume 53, Issue 14
Free
ARVO Annual Meeting Abstract  |   March 2012
Ultrastructural Changes In The Crystalline Lens Of Diabetic Patients Treated With Panretinal Argon Laser Photocoagulation
Author Affiliations & Notes
  • Zeliha Yazar
    Ophthalmology, Ankara Numune Education and Research Hospital, Ankara, Turkey
  • Mustafa Kilic
    Ophthalmology, Ankara Numune Education and Research Hospital, Ankara, Turkey
  • Esra Erdemli
    Histology, Ankara University Medicine Faculty, Ankara, Turkey
  • Ferda Topal Celikkan
    Histology, Ankara University Medicine Faculty, Ankara, Turkey
  • Footnotes
    Commercial Relationships  Zeliha Yazar, None; Mustafa Kilic, None; Esra Erdemli, None; Ferda Topal Celikkan, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science March 2012, Vol.53, 6709. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Zeliha Yazar, Mustafa Kilic, Esra Erdemli, Ferda Topal Celikkan; Ultrastructural Changes In The Crystalline Lens Of Diabetic Patients Treated With Panretinal Argon Laser Photocoagulation. Invest. Ophthalmol. Vis. Sci. 2012;53(14):6709.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: : To investigate the effect of panretinal photocoagulation on the crystalline lens anterior capsula and lens epithelial cells.

Methods: : This prospective, histological, mono-blind study was performed on 20 consecutive cases (20 eyes) who undergone to uncomplicated phacoemulsification surgery for senile nuclear sclerosis (P-1 or less, N-V, C-1 or less according to LOCS). Age of patients varied between 60 to 75 years. They had no complicating systemic or ocular disease except type-2 diabetes mellitus (DM) and hypertension. They were divided into groups according to (1) presence of DM, (2) severity of diabetic retinopathy (DR), and (3)argon laser panretinal photocoagulation (PRP) performed at least 6 months earlier. Lens anterior capsula materials taken from all the patients during phacoemulsification operation were examined with transmission electron microscopy (TEM) by the same treatment-blind histologist. The histo-morphological results were interpreted comparatively.

Results: : All the groups were similar in terms of age and gender distrubution and HbA1c levels in DM (p>0.05 for all).TEM showed no pathological changes in basal membrane in anterior capsule. Filamantous accumulations in the apical surface of the cells were present in nondiabetic control eyes. Sporadic disintegrations in lens epithelial cells (LEC) together with weakening in intercellular connections and mild cell degeneration were observed in eyes with mild nonproliferative DR (NPDR) without PRP. More patchy disintegrations in LEC, weaker intercellular connections, big vacuoles in the cell cytoplasm, picnotic nucleus, and severe degeneration and apoptotic cells with lipid inclusions were noted in eyes with severe NPDR without PRP. In addition to these findings, blebs and surface protrusions compatible with severe degeneration and apoptosis were evident in eyes with PDR and PRP.

Conclusions: : Crystalline lens epithelial cells seems to undergo ultrastructural senescence compatible with the severity of diabetic retinopathy. Argon laser panretinal photocoagulation may accelerate this process through apoptosis.

Keywords: diabetic retinopathy • apoptosis/cell death • cataract 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×