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Takayuki Kamiya, Taiji Nagaoka, Tsuneaki Omae, Ichiro Tanano, Shinji Ono, Akitoshi Yoshida; Sphingosine 1-Phosphate (S1P) Elicits Constriction of Isolated Porcine Retinal Arterioles by Activation of the Rho/ROCK Pathway. Invest. Ophthalmol. Vis. Sci. 2012;53(14):6840.
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© ARVO (1962-2015); The Authors (2016-present)
Sphingosine 1-phosphate (S1P) has been shown to play a role in regulating immune responses, inflammatory processes, and vascular tone, and S1P might be involved in diabetic retinopathy. The purpose of this study was to investigate the effect of S1P on the retinal vessels and whether the Rho/ROCK pathway is involved in S1P-mediated vasoconstriction.
The porcine retinal arterioles (70-102 μm internal diameter) were isolated, cannulated, and pressurized (55 cm H2O) without flow for in vitro study. Videomicroscopic techniques were used to record diameter changes in response to S1P with or without a novel ROCK inhibitor, K115 (Kowa Tokyo, Japan).
Retinal arterioles constricted in response to S1P (0.1 nM-10 μM). The S1P-induced constriction was significantly (P<0.05) reversed by administration of K-115. In addition, pretreatment with K-115 stopped the S1P-induced vasoconstriction.
This study suggested that S1P may elicit constriction of the retinal arterioles via the Rho/ROCK pathway in porcine retinal arterioles.
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