March 2012
Volume 53, Issue 14
Free
ARVO Annual Meeting Abstract  |   March 2012
Different Subsets Of Tumor-infiltrating Lymphocytes Correlate With Macrophage Influx And Monosomy 3 In Uveal Melanoma
Author Affiliations & Notes
  • Inge H. Bronkhorst
    Ophthalmology,
    Leiden University Medical Center, Leiden, The Netherlands
  • T.H. Khanh Vu
    Ophthalmology,
    Leiden University Medical Center, Leiden, The Netherlands
  • Ekaterina S. Jordanova
    Pathology,
    Leiden University Medical Center, Leiden, The Netherlands
  • Gregorius P. Luyten
    Ophthalmology,
    Leiden University Medical Center, Leiden, The Netherlands
  • Sjoerd H. van der Burg
    Clinical Oncology,
    Leiden University Medical Center, Leiden, The Netherlands
  • Martine J. Jager
    Ophthalmology, Leiden University Med Center, Leiden, The Netherlands
  • Footnotes
    Commercial Relationships  Inge H. Bronkhorst, None; T.H. Khanh Vu, None; Ekaterina S. Jordanova, None; Gregorius P. Luyten, None; Sjoerd H. van der Burg, None; Martine J. Jager, None
  • Footnotes
    Support  Stichting Nederlands Oogheelkundig Onderzoek
Investigative Ophthalmology & Visual Science March 2012, Vol.53, 6902. doi:
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      Inge H. Bronkhorst, T.H. Khanh Vu, Ekaterina S. Jordanova, Gregorius P. Luyten, Sjoerd H. van der Burg, Martine J. Jager; Different Subsets Of Tumor-infiltrating Lymphocytes Correlate With Macrophage Influx And Monosomy 3 In Uveal Melanoma. Invest. Ophthalmol. Vis. Sci. 2012;53(14):6902.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : In contrast to many other malignancies, in uveal melanoma, the presence of an immune infiltrate is associated with a bad prognosis. An analysis of the different functional phenotypes of tumor-infiltrating leukocytes (TIL) and a comparison with the genetic background of the tumors may help explain this unusual situation.

Methods: : We performed a comprehensive immunohistochemical study by evaluating the density of CD8+ and CD4+ T lymphocytes, Foxp3+ regulatory T cells (Tregs), CD68+ and CD68+CD163+ macrophages in 43 cases of uveal melanoma. The presence of these different TILs was compared to tumor characteristics.

Results: : The presence of TILs varied greatly between tumors, with among the T cells a predominance of CD8+ T cells, and fewer CD4+ T cells and Tregs. The different subsets of tumor-infiltrating lymphocytes were significantly correlated with each other as well as with the density of M2 macrophages (Spearman correlation coefficient = 0.81), and with the presence of monosomy 3.

Conclusions: : As the numbers of different lymphocytic and myeloid cell types were associated with loss of one chromosome 3, tumor-intrinsic factors probably control the influx of TIL. Their presence may contribute to the increased malignancy seen in high risk uveal melanoma cases.

Keywords: tumors • immunohistochemistry 
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