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Ning Tian, Hui Chen, Ping Wang; Tyrosine Kinase Fyn Regulates The Development Of Retinal Ganglion Cell Dendritic And Axonal Maturation. Invest. Ophthalmol. Vis. Sci. 2012;53(14):6919.
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Fyn, a nonreceptor Src-like tyrosine kinase, plays a pivotal role in neuronal growth in the brain. Blockade of Fyn activity resulted in attenuation of expression of neurofilament, synaptophysin and glutamate receptors in neurons. In developing retina, Fyn is highly concentrated in the differentiating ganglion and amacrine cells. However, the function of Fyn in RGC development is not well investigated. In this study, we examined the role of Fyn on RGC dendritic and axonal maturation.
We qualitatively examined the dendritic ramification in retina and axonal projection in LGN of RGCs of Fyn-/- mice.
Fyn is expressed by RGCs in mouse retina. RGCs of Fyn-/- mice exhibit significantly increased number of dendritic filopodia in developing retina (Fig 1). In addition, Fyn-/- mice showed a significantly reduced spontaneous activity in developing retina. Both the AChR-mediated retinal waves during the first postnatal week and GluR-mediated retinal waves during the second postnatal week have reduced wave size, wave duration, wave strength and firing rates. The increased dendritic filopodia and reduced spontaneous synaptic activity is associated with an impaired RGC axonal projection in the LGN of the Fyn-/- mice. Interestingly, the axonal projections of ipsilateralRGCs are confined in a significantly smaller area in Fyn-/- mice (Fig 2).
Fyn is required for the normal development of RGC dendritic and axonal maturation.
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