Abstract
Purpose: :
Diablo plays an important role in apoptosis cascade. In 2008 ARVO meeting, we reported caspase 3 and caspase 9 siRNA protect the retinal ganglion cell damage induced by transient ischemic injury. We discussed the effects of small interfering RNA (siRNA) targeted against diablo to protect retinal ganglion cells from apoptotic cell death by transient ischemic injury.
Methods: :
Seven week-old male Wister rats were used. We kept on rising IOP 120mmH2O for 45minutes then brought back to normal IOP. SiRNAs designed against rat diablo were injected into the vitreous cavity 24hr prior to transient ischemic injury. Saline was injected in another eyes as contol. Retinal ganglion cells were labeled with Fluoro-Gold retrogradely 3 day before transient ischemia. Three or 8 days after the manipulation, flatmounts of the retina were examined by fluorescence microscopy.
Results: :
In control group, the number of retinal ganglion cell was 720.9(+/-86.4) /mm2 at day 3, 439.9(+/-85.45) /mm2 at day 8. In diablo siRNA-treated groups, the ganglion cell loss significantly inhibited to 1036.1(+/- 88.3)/mm2 in day 3 (p=0.02, Mann-Whitney U test). In day 8 the number of retinal ganglion cell was 790.0(+/- 230.5)/mm2 (p=0.08, Mann-Whitney U test).
Conclusions: :
SiRNA against diablo may protect the retinal ganglion cell damage induced by transient ischemic injury.
Keywords: neuroprotection • RNAi • apoptosis/cell death