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R. S. Chong, Y. Jiang, L. Seet, S. Yu, T. Aung, T. T. Wong; MCP-1 Expression Is Elevated in the Tears and Conjunctiva of Medicated Glaucoma Patients. Invest. Ophthalmol. Vis. Sci. 2009;50(13):463.
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To investigate the cytokine profile in tears and conjunctiva from chronically medicated glaucoma patients.
Tear and conjunctival samples were collected from 53 patients on anti-glaucoma medications due to undergo trabeculectomy. Tears from 22 age-matched, healthy unmedicated subjects served as controls. Tears were collected using Schirmer strips. Conjunctival specimen was retrieved at the time of surgery. Control conjunctiva was collected from subjects undergoing extracapsular cataract extraction on no topical medications. Multiplex bead assay was used to analyse tear cytokines. RTPCR on the conjunctival specimens was used to quantify cytokine expression.
Of the 17 cytokines assayed, MCP-1, IL-6 and IL-8 were consistently detectable in the tears, but only MCP-1 was significantly elevated (P<0.05) in the medicated group relative to control subjects. MCP-1 mRNA expression was elevated at least tenfold in pools of 3 medicated conjunctival specimens analysed by RTPCR compared to the pooled controls.
The use of chronic topical medication increases MCP-1 expression in the tears and the conjunctiva in glaucoma patients. Since MCP-1 is known to be a pro-inflammatory cytokine, its increase may have important implications on the success of subsequent filtration surgery.
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