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K. Schaeferhoff, S. Michalakis, M. Walter, S. Gommel, A. Pravdic, M. Biel, O. Riess, M. Bonin; mRNA Expression Profiling of the Cnga3-/- Mouse With Focus on the Dorsal-Ventral Gradient in Retinal Degeneration. Invest. Ophthalmol. Vis. Sci. 2009;50(13):490.
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© ARVO (1962-2015); The Authors (2016-present)
The CNGA3-/- mouse is an animal model lacking the A subunit of the cone specific cyclic nucleotide gated channel. The phenotype is characterized by a loss of cone photoreceptor function and a progressive degeneration of the cones, particularly in the ventral part of the retina. To elucidate this dorsal ventral gradient in degeneration we performed whole genome expression analyses of dorsal and ventral areas of CNGA3-/- and wildtype retinas at the age of 4 weeks.
Expression analysis of CNGA3-/- and wildtype retinas was performed using Illumina MouseWG-6 v1.0 Expression BeadChips. Differential regulated transcripts with a minimum change in expression level of 1.5 fold (p-value ≤0.05) were obtained and gene regulation networks were generated by the Ingenuity Pathways Analysis software. To verify the data several transcripts were analyzed by qRT-PCR. The study was performed in accordance with the ARVO Statement for the use of Animals in Ophthalmic and Visual Research.
Physiological differences in expression patterns of the dorsal and ventral retina were analyzed in wildtype samples, showing 264 differently regulated transcripts. Differences between the wildtype and CNGA3-/- retinas appeared with 579 differently regulated transcripts in the dorsal areas and 608 in the ventral retina. In contrast to the wildtype, CNGA3-/- mice showed a differently regulation of 227 transcripts in the ventral retina. The results could be successfully verified by qRT PCR.
Expression analysis of different retinal areas showed clear differences in expression patterns of the dorsal and ventral regions. Analysis in CNGA3-/- retinas discovered clear differences in expression patterns. These results could help to elucidate the molecular events leading to the dorsal-ventral gradient of cone degeneration in CNGA3-/- mice.
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