Purchase this article with an account.
M. Esmaeelpour, B. Povaay, F. Bounaparte, B. Hofer, B. Hermann, A. R. Tumlinson, R. V. North, S. L. Hale, N. J. L. Sheen, W. Drexler; In vivo Human Choroidal Thickness Measurements Using Three-Dimensional High Resolution OCT at 1060 nm. Invest. Ophthalmol. Vis. Sci. 2009;50(13):1090.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
To measure choroidal thickness in healthy subjects using three-dimensional OCT at 1060 nm and to examine the relationship with axial length.
Three-dimensional (3D) OCT at 1060 nm with about 6-8 µm axial resolution and 47.000 A-scans/second resulting in up to 70 frames/second (with each 512 A-scans) has been used. Wide-field 3D OCT over about 30 degree centred on the fovea was acquired without pupil dilation using in 30 eyes. Subject age ranged from 20 to 80 years (median 42 years). In addition, five axial length measurements were averaged from each eye using optical biometry (IOL master (Zeiss, Jena, Germany). Central choroidal thickness (C) was determined by averaging transversally over a 1x1 degrees angular field to compensate for individual choroidal vessels between the choroidal/ RPE and choroid/ scleral boundary using Image J software (freeware). Thickness was further determined for the nasal (N), temporal (T), superior (S) and inferior (I) choroid at a distance of 7.5 degrees to the fovea centre.
Central choroidal thickness ranged from 133 to 487µm (mean 312, SD 105). Choroidal structure details were visible over an area of 25-30 degrees. Mean results (SD) in µm from non-central choroids were: N 176 (74), T 200 (75), S 265 (115), I 273 (101). Axial length measurements ranged from 22.3 to 31.7mm (mean 24.9, SD 2.5). A statistical significant correlation with a p<0.05 for all measured points (Pearson’s, Cr= -0.74, Nr= -0.6, Tr= -0.62, Sr= -0.6 and Ir= -0.64) indicates that choroidal thickness decreases with increasing axial length.
For the first time, direct in vivo visualisation and quantification of the human choroidal thickness in normals using 3D OCT at 1060 nm has been successfully carried out and its correlation to axial eye length could be determined over a large area. Quantification of healthy choroidal structure may serve determining how the choroid is affected in different conditions which could indicate early changes in diabetes retinopathy, glaucoma or age related macula degeneration.
This PDF is available to Subscribers Only