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J. J. Liu, B. Potsaid, Y. Chen, I. Gorczyska, V. J. Srinivasan, J. S. Duker, J. G. Fujimoto; Ultrahigh Resolution Spectral / Fourier Domain OCT Imaging of the Rat Retina at 70,000 Axial Scans Per Second. Invest. Ophthalmol. Vis. Sci. 2009;50(13):1111.
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To demonstrate non-invasive in vivo rat retinal imaging using ultrahigh resolution spectral / Fourier domain optical coherence tomography (OCT) at 70,000 axial scans per second and ~3 µm axial resolution. The high speed and high density data sets enable improved en face visualization by reducing eye motion artifacts and improve Doppler OCT measurements.
An ultrahigh resolution spectral / Fourier domain OCT prototype instrument has been developed for small animal imaging using new high speed CMOS imaging technology. This technology can achieve imaging speeds over 70,000 axial scans per second and axial image resolutions of ~3 µm. A microscope delivery system was used for focusing and scanning the OCT beam in the animal eye. Three dimensional OCT (3D-OCT) data sets of the rat retina were acquired. OCT fundus images were created from 3D-OCT data. Doppler OCT analysis of blood flow in the rat retina was performed.
OCT imaging of the rat retina was performed at 70,000 axial scans per second with ~3 µm resolution. An 3D-OCT data set containing 180 images, each consisting of 512 axial scans, is acquired in ~1.5 seconds. Minimal motion artifacts are visible and the OCT fundus images offer more precise registration of individual OCT images to retinal fundus features. Projection OCT fundus images show features such as the nerve fiber layer, retinal capillary networks and choroidal vasculature. Doppler OCT images and quantitative measurements show pulsatility in retinal blood vessels.
Ultrahigh resolution retinal imaging was demonstrated in the rat using spectral / Fourier domain OCT. 3D-OCT data sets obtained at high speed show reduced motion artifacts, enabling improved en face OCT fundus imaging. Doppler OCT provides non-invasive in vivo quantitative measurements of retinal blood flow properties and may benefit studies of diseases such as glaucoma and diabetic retinopathy. Ultrahigh resolution spectral / Fourier domain OCT promises to enable novel protocols for measuring small animal retinal structure and retinal blood flow.
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