April 2009
Volume 50, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2009
Ultrahigh Resolution Spectral / Fourier Domain OCT Imaging of the Rat Retina at 70,000 Axial Scans Per Second
Author Affiliations & Notes
  • J. J. Liu
    Department of Electrical Engineering and Computer Science, and Research Laboratory of Electronics, Massachusetts Institute of Technology, Cambridge, Massachusetts
  • B. Potsaid
    Department of Electrical Engineering and Computer Science, and Research Laboratory of Electronics, Massachusetts Institute of Technology, Cambridge, Massachusetts
    Advanced Imaging Group, Thorlabs, Inc., Newton, New Jersey
  • Y. Chen
    Department of Electrical Engineering and Computer Science, and Research Laboratory of Electronics, Massachusetts Institute of Technology, Cambridge, Massachusetts
    New England Eye Center, Tufts Medical Center, Boston, Massachusetts
  • I. Gorczyska
    Department of Electrical Engineering and Computer Science, and Research Laboratory of Electronics, Massachusetts Institute of Technology, Cambridge, Massachusetts
    New England Eye Center, Tufts Medical Center, Boston, Massachusetts
  • V. J. Srinivasan
    Department of Electrical Engineering and Computer Science, and Research Laboratory of Electronics, Massachusetts Institute of Technology, Cambridge, Massachusetts
  • J. S. Duker
    New England Eye Center, Tufts Medical Center, Boston, Massachusetts
  • J. G. Fujimoto
    Department of Electrical Engineering and Computer Science, and Research Laboratory of Electronics, Massachusetts Institute of Technology, Cambridge, Massachusetts
  • Footnotes
    Commercial Relationships  J.J. Liu, None; B. Potsaid, Thorlabs, Inc., E; Y. Chen, None; I. Gorczyska, None; V.J. Srinivasan, None; J.S. Duker, Carl Zeiss Meditec, Inc., F; Optovue Corporation, F; Topcon, F; J.G. Fujimoto, Optovue Corporation, I; Carl Zeiss Meditec, Inc., P.
  • Footnotes
    Support  NIH R01-EY11289-21, NIH R01-EY13178-07, NIH R01-CA75289-11, NSF BES-0522845; AFSOR FA9550-07-1-0014 and MFEL FA9550-07-1-0101
Investigative Ophthalmology & Visual Science April 2009, Vol.50, 1111. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      J. J. Liu, B. Potsaid, Y. Chen, I. Gorczyska, V. J. Srinivasan, J. S. Duker, J. G. Fujimoto; Ultrahigh Resolution Spectral / Fourier Domain OCT Imaging of the Rat Retina at 70,000 Axial Scans Per Second. Invest. Ophthalmol. Vis. Sci. 2009;50(13):1111.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: : To demonstrate non-invasive in vivo rat retinal imaging using ultrahigh resolution spectral / Fourier domain optical coherence tomography (OCT) at 70,000 axial scans per second and ~3 µm axial resolution. The high speed and high density data sets enable improved en face visualization by reducing eye motion artifacts and improve Doppler OCT measurements.

Methods: : An ultrahigh resolution spectral / Fourier domain OCT prototype instrument has been developed for small animal imaging using new high speed CMOS imaging technology. This technology can achieve imaging speeds over 70,000 axial scans per second and axial image resolutions of ~3 µm. A microscope delivery system was used for focusing and scanning the OCT beam in the animal eye. Three dimensional OCT (3D-OCT) data sets of the rat retina were acquired. OCT fundus images were created from 3D-OCT data. Doppler OCT analysis of blood flow in the rat retina was performed.

Results: : OCT imaging of the rat retina was performed at 70,000 axial scans per second with ~3 µm resolution. An 3D-OCT data set containing 180 images, each consisting of 512 axial scans, is acquired in ~1.5 seconds. Minimal motion artifacts are visible and the OCT fundus images offer more precise registration of individual OCT images to retinal fundus features. Projection OCT fundus images show features such as the nerve fiber layer, retinal capillary networks and choroidal vasculature. Doppler OCT images and quantitative measurements show pulsatility in retinal blood vessels.

Conclusions: : Ultrahigh resolution retinal imaging was demonstrated in the rat using spectral / Fourier domain OCT. 3D-OCT data sets obtained at high speed show reduced motion artifacts, enabling improved en face OCT fundus imaging. Doppler OCT provides non-invasive in vivo quantitative measurements of retinal blood flow properties and may benefit studies of diseases such as glaucoma and diabetic retinopathy. Ultrahigh resolution spectral / Fourier domain OCT promises to enable novel protocols for measuring small animal retinal structure and retinal blood flow.

Keywords: imaging methods (CT, FA, ICG, MRI, OCT, RTA, SLO, ultrasound) • imaging/image analysis: non-clinical • retina 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×