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W. Chen, Y. Lin, L. Wang, M. Liu, Y. Ye, J. Qu; Comparison of Fresh Corneal Tissue versus Glycerin-Cryopreserved Corneal Tissue in Deep Anterior Lamellar Keratoplasty. Invest. Ophthalmol. Vis. Sci. 2009;50(13):628.
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© ARVO (1962-2015); The Authors (2016-present)
To compare microstructural differences in fresh corneal tissue with glycerin-cryopreserved corneal tissue (GCCT) used during deep anterior lamellar keratoplasty (DALK).
The medical records of 48 patients who underwent DALK for stromal opacity without endothelial abnormalities were retrospectively reviewed. Patients were divided into 2 groups according to corneal tissue used, a fresh corneal tissue group (n=22) and a GCCT group (n=26). Slit-lamp, corneal topography, pachymetry, and laser scanning in vivo confocal microscopy examinations were performed at 2 weeks and 1, 3, 6, 12, and 24 months postoperatively..
No rejection developed in the GCCT group. Stromal rejection developed in one eye (4.2%) in the fresh corneal tissue group. In 19 eyes (73.1%) in the GCCT group, the best-corrected visual acuity (BCVA) was 6/12 or better compared with 17 eyes (77.3%) in the fresh corneal tissue group at 24 months. The median spherical equivalents in the GCCT and fresh corneal tissue groups were, respectively, -2.46 and -1.81 at the final visit. There were no significant differences in BCVA, spherical equivalent, astigmatism, central corneal thickness, or endothelial cell density between the groups at 24 months. While progressive significant (P<0.05) reductions in keratocyte density in the central stroma developed at each examination in the fresh corneal tissue group, the GCCT group had progressively increasing keratocyte density from 3 months, but no cellular structures were observed at 2 weeks and 1 month. Confocal scanning showed only subbasal nerve fiber bundles in the fresh corneal tissue group (10 eyes) and the GCCT group (14 eyes) until 24 months postoperatively.
DALK using GCCT provides results comparable to fresh tissue. GCCT can be used safely and effectively for DALK and may minimize stromal rejection postoperatively.
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