April 2009
Volume 50, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2009
Inhibitory Effect of Choroidal Neovascularization in Rat by Systemic Administration of Protein Kinase C Inhibitor, Benzthiazole Derivatives
Author Affiliations & Notes
  • J. Chae
    Department of Ophthalmology,
    University of Ulsan, College of Medicine, Asan Medical Center, Seoul, Republic of Korea
  • K. Cho
    Department of Ophthalmology,
    University of Ulsan, College of Medicine, Asan Medical Center, Seoul, Republic of Korea
  • J. Lee
    Department of Ophthalmology,
    University of Ulsan, College of Medicine, Asan Medical Center, Seoul, Republic of Korea
  • J.-G. Kim
    Department of Ophthalmology,
    University of Ulsan, College of Medicine, Asan Medical Center, Seoul, Republic of Korea
  • J. Koh
    Department of Neurology,
    University of Ulsan, College of Medicine, Asan Medical Center, Seoul, Republic of Korea
  • Y. Yoon
    Department of Ophthalmology,
    University of Ulsan, College of Medicine, Asan Medical Center, Seoul, Republic of Korea
  • Footnotes
    Commercial Relationships  J. Chae, None; K. Cho, None; J. Lee, None; J.-G. Kim, None; J. Koh, None; Y. Yoon, None.
  • Footnotes
    Support  This study was supported by a grant of the Korea Healthcare technology R&D Project, Ministry of Health, Welfare & Family Affairs, Republic of Korea. A080557
Investigative Ophthalmology & Visual Science April 2009, Vol.50, 789. doi:
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      J. Chae, K. Cho, J. Lee, J.-G. Kim, J. Koh, Y. Yoon; Inhibitory Effect of Choroidal Neovascularization in Rat by Systemic Administration of Protein Kinase C Inhibitor, Benzthiazole Derivatives. Invest. Ophthalmol. Vis. Sci. 2009;50(13):789.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To investigate the inhibitory effect of intraperitoneally administered Benzthiazole derivative, Protein Kinase C inhibitor, in laser induced choroidal neovascularization of rat.

Methods: : In 36 eyes of 36 Female Brown Norway Rats, CNV was induced by Argon laser photocoagulation at the onset of experiment. They are divided into three groups: 10mg/kg Benzthiazole derivative with DMSO injection group, only DMSO injection group, and no treatment group (control). After 14 days, Fluorescein angiography (FA) was performed, and the amount of fluorescein leakage was assessed by Clinical score. All the eyes were enucleated at the next day after FA, and the thickness of choroidal neovascularization was measured by Hematoxylin and Eosin Stain. Also, VEGF (vascular endothelial growth factor), and PDGF (platelet derived growth factor) were assessed immunohistochemically.

Results: : In Benzthiazole derivate injection group, Choroidal neovascularization was significantly reduced on clinical score of FA (P-value<0.05). The CNV thickness of Benzthiazole derivative treatment group was thinner than Control group (pure DMSO injection group, and no treatment group) on Hematoxylin and Eosin stain. On immunohistochemistry stain, VEGF and PDGF were less expressed at Benzthiazole derivative treatment group.

Conclusions: : Benzthizole derivative can reduce the laser induced choroidal neovascularization of rat, and may be useful in treatment of CNV.

Keywords: choroid: neovascularization • vascular endothelial growth factor • age-related macular degeneration 
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