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Y. You, G. Galicias-Rosas, S. Van de Veire, T. Van Bergen, J. Ceuppens, I. Stalmans, R. Van Ginderdeuren; Haptoglobin Knockout Mice Exhibit a Form of Developmental Glaucoma and Delay of Lens Maturation, Linked to Diminished C-MAF Expression. Invest. Ophthalmol. Vis. Sci. 2009;50(13):865.
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To study the ocular phenotype of mice that lack Haptoglobin (Hpko) and investigate whether these changes correlate with the expression of C-maf. C-maf is known to be expressed in embryonic life and to be involved in the development of the lens and anterior segment.
Hpko mice and wild type(WT)littermates were histological investigated at embryonic day(E)12,15 and 18 and postnatal day(P)1,4 and 21. The expression of C-maf was studied at mRNA level by rt-PCR. C-maf expression in eyes was compared in adult mice between clinically blind versus not-blind Hpko and WT mice and in embryos at E12,between offspring from clinical blind versus not-blind Hpko mothers.
Eyes of Hpko mice were histological normal at E12 compared with WT mice. At P1 eyes of Hpko mice were significantly smaller (mean 29%); in 55% of mice the lens was not yet separated form the cornea. At P21 12% of the eyes from Hpko mice still showed contact between lens and cornea; anterior senychiae were present in 42% of the eyes, whereas these structural abnormalities were never observed in WT eyes.The ocular C-maf mRNA expression at E12 was significantly lower in Hpko embryos as compared to WT embryos (P<0.05). C-maf expression levels in eyes of Hpko non-blind mothers were intermediate.
Haptoglobin knockout mice exhibit developmental disorders that are similar to those observed in developmental glaucoma. There is a delay in development of the anterior segment structures, which start during late embryonic life and continue after birth. C-maf expression is diminished at E12 and this may be linked to the aberrant anterior chamber development.
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