April 2009
Volume 50, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2009
Properties of Recovered Visual Function in the Channelrhodopsin-2 Transferred Royal College of Surgeons Rats
Author Affiliations & Notes
  • H. Tomita
    International Adv Interdisciplinary Res, Tohoku University, Sendai, Japan
  • E. Sugano
    International Adv Interdisciplinary Res, Tohoku University, Sendai, Japan
  • H. Isago
    International Adv Interdisciplinary Res, Tohoku University, Sendai, Japan
  • T. Hiroi
    International Adv Interdisciplinary Res, Tohoku University, Sendai, Japan
  • H. Yawo
    Department of Developmental Biology and Neuroscience, Tohoku University Graduate School of Life Sciences, Sendai, Japan
  • M. Tamai
    Tohoku University Graduate School of Medicine, Sendai, Japan
  • Footnotes
    Commercial Relationships  H. Tomita, None; E. Sugano, None; H. Isago, None; T. Hiroi, None; H. Yawo, None; M. Tamai, None.
  • Footnotes
    Support  KAKENHI (19659442, 20791241), JRPS, Suzuken Foundation
Investigative Ophthalmology & Visual Science April 2009, Vol.50, 981. doi:
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      H. Tomita, E. Sugano, H. Isago, T. Hiroi, H. Yawo, M. Tamai; Properties of Recovered Visual Function in the Channelrhodopsin-2 Transferred Royal College of Surgeons Rats. Invest. Ophthalmol. Vis. Sci. 2009;50(13):981.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To test the hypothesis that a transduction of the channelrhodopsin-2 gene, a microbial-type rhodopsin gene, into retinal ganglion cells of genetically blind rats will restore functional vision.

Methods: : We made an adeno-associated virus vector type 2 including the channelrhodopsin-2 gene fused to a fluorescent protein, Venus (AAV2-ChR2V). The AAV-ChR2V was injected intravitreally into the eyes of 6-month-old RCS (rdy/rdy) rats. Visual function was evaluated periodically by recording visually evoked potentials (VEPs) with various stimulus patterns and optomotor responses. The expression of ChR2V in the retina was investigated histologically.

Results: : VEPs could not be recorded from 6-month-old untreated RCS rats. In contrast, VEPs were markedly elicited from RCS rats injected with AAV-ChR2V at 6 weeks post-injection. The VEPs of RCS rats injected with AAV-ChR2V were first detected at 2 weeks after the injection. Thereafter, the amplitude progressively increased up to 6 weeks post-injection. The RCS rats with ChR2V and non dystrophic rats did not respond to 40 Hz and 50 Hz stimuli frequencies. However, VEPs were detected with stimuli <20 Hz of light simulation in both types of rats. The optomotor responses were significantly stronger after the AAV2-ChR2V injection. The expression of ChR2V was observed mainly in the retinal ganglion cells.

Conclusions: : These findings demonstrate that blind rats can regain their visual function by the transduction of the ChR2V gene into the retinal ganglion cells.

Keywords: retinitis • gene transfer/gene therapy • regeneration 
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