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M. Kim, J. Kim, J. Kim, K.-H. Park, K.-W. Kim, Y. Yu; Differential Expression of Stem Cell Markers and VEGF in Human Retinoblastoma Tissue. Invest. Ophthalmol. Vis. Sci. 2009;50(13):1685.
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© ARVO (1962-2015); The Authors (2016-present)
To investigate whether VEGF contributes to the development of the cancer stem cell-vascular niche complex in human retinoblastoma tissue.
Using six human retinoblastoma specimens which were primarily enucleated for Reese-Ellsworth classifiacation stage 5a, immunofluorescence for ABCG2 for the cancer stem cell marker, MCM2 for the neural stem cell marker and VEGF for the angiogenic cytokine was performed. To compare the localization of them, double immunofluorescence was done as well.
In normal retina, ABCG2 and MCM2 were sporadically expressed in the ganglion cell layer (GCL) and the inner nuclear layer (INL), whereas VEGF was diffusely expressed in inner retina where retinal vessels are abundantly distribute. In the tumor, ABCG2 was strongly expressed out of Wintersteiner rosettes, whereas MCM2 and VEGF were strongly stained in the rosettes. Interestingly, MCM2 was positive in the outer portion of the rosettes, whereas VEGF was positive in the inner portion.
Our data demonstrated that MCM2 and VEGF are strongly expressed in the rosettes of tumor, which were far from the area of ABCG2-positive cells. Although VEGF might not directly contribute to cancer stem cell-vascular niche complex, it could play some role in differentiation of tumor cells to build up the rosettes.
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