Purchase this article with an account.
N. Koizumi, N. Okumura, Y. Sakamoto, H. Takahashi, K. Hirata, N. Okahara, H. Tsuchiya, R. Torii, T. Inatomi, S. Kinoshita; Cultivated Monkey Corneal Endothelial Cell Transplantation Using Corneal Lamellar Graft. Invest. Ophthalmol. Vis. Sci. 2009;50(13):1811.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
Previously, we established a primate animal model of cultivated corneal endothelial transplantation using cynomolgous monkeys whose corneal endothelial cells have low proliferative ability (Koizumi, et al. IOVS 2007). The present study was designed to investigate the feasibility of cultivated corneal endothelial cell transplantation using a corneal lamellar graft in animal eyes with corneal endothelial dysfunction.
The experimental protocol was approved by the regulations of university animal care and use committees, and animals were handled in accordance with ARVO guidelines. Descemet’s membrane with intact corneal endothelial cells was removed from human corneal eye bank tissue. Corneal lamellar grafts (150µm thickness and 8mm diameter) were made from the posterior stroma with a Moria microkeratome. Cultivated monkey corneal endothelial cells (MCECs) were seeded on the human corneal lamellar grafts and cultivated for 3 weeks. Under general anesthesia, the corneal endothelia and Descemet’s membrane of 2 eyes of 2 animals (1 rabbit and 1 monkey) were removed, and the grafts with MCECs were transplanted using Busin glide in a similar procedure to Descemet-stripping automated endothelial keratoplasty (DSAEK).
Confluent MCECs were established on the graft with a density of 2200 cells/mm2 and expressed ZO-1 and Na+-K+ ATPase. The day after surgery, grafts were well attached to the host corneal stroma and mild corneal edema was observed. Histological examination of the rabbit eye 2 days after surgery showed no donor endothelial damage due to the graft insertion. The monkey cornea recovered its clarity by 2 weeks postoperatively. Two months after surgery, the monkey cornea remained clear, was 750µm thick, with hexagonal cells observed by specular microscopy at a density of 2200 cells/mm2. No signs of rejection were detected.
We cultivated MCECs on a human corneal lamellar graft and successfully transplanted this construct into animal eyes with the DSAEK technique. Our results suggest the possibility of cultivated corneal endothelial cell transplantation using a corneal lamellar graft.
This PDF is available to Subscribers Only