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E. C. Andersen, J. R. Andreasen, K. A. Poulsen, N. Junker, M. H. Nissen, M. Kolko; Cross-Talk Between Vascular Endothelial Growth Factor and Calcium-Independent Phospholipase A2 in the Regulation of Retinal Pigment Epithelium Cell Migration and Proliferation. Invest. Ophthalmol. Vis. Sci. 2009;50(13):1853.
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During pathological conditions the retinal pigment epithelium (RPE) cells can be triggered to proliferate and migrate.Vascular endothelial growth factor (VEGF) and calcium-independent phospholipase A2, group IVA (iPLA2-VIA) have been shown to be involved in cell proliferation and migration1,2. The purpose of this study was to evaluate the role of iPLA2-VIA in migration of RPE cells and to investigate a possible cross talk between VEGF and iPLA2-VIA.
ARPE-19 cells were transfected with an iPLA2-VIA promoter-luciferase constructs to elucidate the regulation of iPLA2-VIA after exposure to VEGF. Western blot analysis was performed to investigate the expression of VEGF in cells transfected with an iPLA2-VIA construct. Proliferation of ARPE-19 cells was evaluated by incorporation of radioactively labeled thymidine and migration was evaluated by the use of a Boyden chamber. VEGF was added to cells and effect of iPLA2-VIA was examined by exposing the cells for commercial iPLA2 inhibitors.
The present study reveal an upregulation of the promoter gene for iPLA2-VIA as well as an upregulation of iPLA2-VIA-protein in ARPE-19 cells treated with VEGF. Exposure of VEGF stimulates ARPE-19 cells to proliferate and migrate. Both proliferation and migration can be reduced by iPLA2-inhibitors.
The present results show a cross talk between VEGF and iPLA2-VIA and indicate a role of iPLA2-VIA in the regulation of RPE proliferation and migration.
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