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N. S. Dejneka, J. M. Nigro, O. S. Bond, N. K. Shams; Cytokine Profile of ARPE19 Cells: Lack of Production of IFN-, IFN-β and IFN- Following Stimulation with Poly (I:C). Invest. Ophthalmol. Vis. Sci. 2009;50(13):1867.
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To characterize the cytokine secretion profile of ARPE19 cells following treatment with polyinosinic-polycytidylic acid sodium salt [Poly (I:C)], a dsRNA analogue.
ARPE19 cells were seeded in 24 well plates (50,000 cells per well). Twenty-four hours later, media was removed and cells were treated with Poly (I:C); 0-1000 µg/mL or poly deoxyinosinic-deoxycytidylic acid sodium salt [Poly (dI:dC); 50mU/mL], prepared in serum free media. Twenty-four hours post-treatment, media was collected from cells and analyzed for IFN-, IFN-β, IFN-γ, IL-8, IL-6, TNF-, ICAM and MCP-1 via ELISA. Cell cytoxicity was determined via the alamarBlue® Assay.
Poly (I:C) and Poly (dI:dC) showed no cytotoxicity at the doses tested. ARPE19 cells did not produce IFN-, IFN-β or IFN-γ. Cells produced IL-8, IL-6, TNF-, ICAM and MCP-1 in a dose-dependent manner.
ARPE19 cells produce several inflammatory cytokines but do not produce three key mediators, IFN-, IFN-β or IFN-γ. These cells can be used to study the inflammatory potential and specific effects of dsRNAs such as siRNAs.
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