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C. A. Medina-Mendez, R. E. Martinez, A. Sidani, G. Amescua, Y. Tan, G. Puig, V. L. Perez; Role of T Cell Chemoattractants CXCL10/IP10 and CXCL9/Mig in High Risk Corneal Allograft Transplantation. Invest. Ophthalmol. Vis. Sci. 2009;50(13):1977.
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We have previously reported that the T cell chemoattractants, CXCL10/IP10 and CXCL9/Mig, are up regulated in high risk corneal allografts after transplantation. The goal of this work is to understand how these regulate the kinetics of graft rejection.
Fully MHC mismatched corneal allografts (Balb/c to C57BL6) were performed in vascularized high risk recipients treated with anti-CXCL9 or anti CXCL10 antibody or control. Graft survival was monitored and quantification of infiltrating inflammatory cells was performed by immunohistochemistry. T cell priming data was also gathered by ELISPOT.
High risk corneal allograft rejection associated with the neutralization of CXCL10 correlated with an increased recruitment of macrophages and T cells into the corneal allograft. Neutralization of CXCL10/IP10 induced graft rejection was not associated with differences in T cell priming. Interestingly and similar to CXCL10/IP10 neutralization data, neutralization of the other produced T cell chemoattractant CXCL9/Mig, resulted in increased high risk corneal allograft rejection.
Our data suggests that the role of T cell chemoattractant production by high risk corneal allografts may have a regulatory effect on the recrutiment of T cells that results in graft protection rather than rejection.
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