April 2009
Volume 50, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2009
Expression of Dominant Negative K6w-Ubiquitin in Lens Epithelium Using Adenoviral Vector Delays Posterior Capsular Opacification in a Rabbit Model
Author Affiliations & Notes
  • M. Wu
    Cataract, Zhongshan Ophthalmic Center, Guangzhou, China
  • R. Peng
    Department of Ophthalmology,, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, China
  • Y. Zhao
    Cataract, Zhongshan Ophthalmic Center, Guangzhou, China
  • Z. Liu
    Cataract, Zhongshan Ophthalmic Center, Guangzhou, China
  • X. Liu
    Cataract, Zhongshan Ophthalmic Center, Guangzhou, China
  • F. Shang
    Laboratory for Nutrition and Vision Research, Jean Mayer USDA HNRA at Tufts University., Boston, Massachusetts
  • Footnotes
    Commercial Relationships  M. Wu, None; R. Peng, None; Y. Zhao, None; Z. Liu, None; X. Liu, None; F. Shang, None.
  • Footnotes
    Support  Chinese NSFC 30572003
Investigative Ophthalmology & Visual Science April 2009, Vol.50, 2128. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      M. Wu, R. Peng, Y. Zhao, Z. Liu, X. Liu, F. Shang; Expression of Dominant Negative K6w-Ubiquitin in Lens Epithelium Using Adenoviral Vector Delays Posterior Capsular Opacification in a Rabbit Model. Invest. Ophthalmol. Vis. Sci. 2009;50(13):2128.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: : To evaluate the effects of expression of dominant negative K6W-ubiquitin in lens epithelium using adenoviral vector on epithelial-mesenchymal transition and the formation of posterior capsular opacification (PCO) in a rabbit model.

Methods: : Twenty four rabbits were divided into 4 groups randomly. Phacoemulsification was performed on both eyes. After water-tight suturation of scleral turnel incision, different quantities of recombinant dominant negative K6W-ubiquitin adenovirus and empty virus (group A, 2.82×105ifu/mL; group B, 1.13×106ifu/mL; group C, 2.26×106ifu/mL; and group D, 4.29×106ifu/mL) was diluted to 0.5mL and injected into right and left anterior chambers, respectively. Posterior capsular opacification were assessed and documented by slit lamp photography at 1, 3, 5, 7, 11 and 15 days after surgery. The degree of PCO was calculated by posterior capsule opacification manual software (POCOman). Two weeks after operation, lens capsules were dissected for immunofluorescenct detection of -SMA.

Results: : In group A, there is no statistical difference in degrees of PCO between the K6W-ubiquitin-expressed and control eyes. In group B, the degrees of PCO in K6W-ubiquitin-expressed eyes were lower than those in the control eyes at 11 and 15 days after surgery. In group C, the protective effects were observed at 5,7,11 and 15 days after surgery. In group D, the protective effects of K6W-ubiquitin expression on PCO were detected, however, severe corneal edema was also observed. The expression of -SMA in the K6W-ubiquitin group was lower than that of the control group.

Keywords: cataract • cell survival • drug toxicity/drug effects 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×