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N. Tojo, Y. Kashiwagi, T. Yamamoto, S. Yamamoto, H. Yamashita; Effects of Cytokines and Chemically-Active Agents on Interaction Between Vitreou-Derived Hyalocytes and Vascular Endothelial Cells. Invest. Ophthalmol. Vis. Sci. 2009;50(13):2706.
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© ARVO (1962-2015); The Authors (2016-present)
Ocular angiogenesis is regulated by a number of polypeptides including cytokines and growth factors which are known to affect vascular endothelial cell functions. In the previous reports, we have shown interactions between vitreous-derived cells (hyalocytes) and endothelial cells, and certain effects of cytokines on the above cells.
To determine the effects of various chemical agents on the viability of endothelial cells in the co-culture system with porcine hyalocytes. The viability of human retinal endothelial cells (HRECs) with or without porcine hyalocytes was measured by MTT assay in the presence of IL-1, IL-1β, IL-6, TNF or VEGF. These effects were compared with the viability of HRECs co-cultured with porcine hyalocytes. We examined the effects of bevacizumab, fenofibrate, and dexamethasone.
The viability of HRECs was increased by exposure to IL-1, IL-1β, IL-6, TNF or VEGF. The viability of HRECs was also increased by the co-culture with porcine hyalocytes in the presence of IL-1, IL-1β, IL-6, TNF or VEGF. Bevacizumab decreased the viability of HRECs stimulated by VEGF without porcine hyalocytes at 10µg/ml, and the effect of bevacizumab to block VEGF fucntions was decreased in the presence of hyalocytes 100µg/ml of bevacizumab was required to block VEGF effects. Fenofibrate (a lipid-modifying agent: 5 µg/ml) decreased the viability of HRECs stimulated by IL-1β or VEGF without hyalocytes, and did not decrease the viability of HRECs with hyalocytes. Dexamethasone (50µg/ml) decreased the viability of HRECs stimulated by IL-1, IL-1β, IL-6 or VEGF without hyalocytes, and did not decrease the viability of HRECs with hyalocytes.
HRECs decreased the effects of agents (bevacizumab, fenofibrate or desamethozone) in the presence with porcine hyalocytes. These results suggest that the co-culture system is a good candidate to evaluate effects of bio-acitve agents on the cultured cells.
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