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H. Kyung, K. Park, Y. Kim, J. Jeoung, M. Seo, H. Park, M. Park, S. Kim; Mitochondrial Enhancement in RGC and IOP Reduction by MB660 in DBA/2J Mouse. Invest. Ophthalmol. Vis. Sci. 2009;50(13):2782.
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© ARVO (1962-2015); The Authors (2016-present)
MB660 is derived from natural compound. This material has neuroprotective effects by enhancing the ratio of NAD+/NADH in the mitochondria. We investigated the neuroprotective effect on retinal ganglion cells(RGC) in DBA/2J mouse.
From a preliminary study and dose-dependent study in C57BL/6J mouse, daily experimental MB660 dosage of low(30mg/kg) and high(60mg/kg) dose were determined. Main experiment animals were 7-month-old, male, DBA/2J mice. In the control group, normal diet was applied. In experiment groups, low MB660 diet(low dose group) or high MB660 diet(high dose group) was applied. After 4 weeks later, right eyes were flat-mounted for RGC counting 24hr after DTMR labeling, and left eyes were prepared for axon counting by Bodian staining. Also the number, distribution and shape of the mitochondria were investigated by the transmission EM(TEM) view of the optic nerve in the left eyes. IOP was measured by the rebound tonometer at the first time and 4 weeks later.
The difference of RGC and axon counts were not statistically significant among three groups. IOP was increased in the control group(21.66 ± 6.49 --> 23.60 ± 7.38 (n=50), p=0.181), but IOP of high dose group was decreased significantly(21.55 ± 5.80 --> 18.88 ± 5.00 (n=40), p=0.017). IOP of low dose group showed the tendency of IOP reduction, however, statistically not significant(21.16 ± 5.97 --> 19.33 ± 6.27 (n=48), p=0.082). TEM view revealed increased number and bigger size of the mitochondria in the experiment groups than control group.
Four-week-experiment could not confirm neuroprotective effects of MB660 in DBA/2J mice. However, the enhancement of mitochondria and IOP reduction imply future potential of MB660 to be applied in glaucoma treatment.
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