April 2009
Volume 50, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2009
Gene Targeting to Murine Retinal Bipolar Cells
Author Affiliations & Notes
  • L. J. Stein
    FM Kirby Ctr Molecular Ophth/Scheie Eye Inst, University of Pennsylvania, Philadelphia, Pennsylvania
  • D. C. Chung
    FM Kirby Ctr Molecular Ophth/Scheie Eye Inst, University of Pennsylvania, Philadelphia, Pennsylvania
  • J. Bennicelli
    FM Kirby Ctr Molecular Ophth/Scheie Eye Inst, University of Pennsylvania, Philadelphia, Pennsylvania
  • Z. Wei
    FM Kirby Ctr Molecular Ophth/Scheie Eye Inst, University of Pennsylvania, Philadelphia, Pennsylvania
  • J. Bennett
    FM Kirby Ctr Molecular Ophth/Scheie Eye Inst, University of Pennsylvania, Philadelphia, Pennsylvania
  • Footnotes
    Commercial Relationships  L.J. Stein, None; D.C. Chung, None; J. Bennicelli, None; Z. Wei, None; J. Bennett, None.
  • Footnotes
    Support  NIH Grants K08 EY017024, RO1 EY10820, P30-DK-47747-10, Foundation Fighting Blindness, Research to Prevent Blindness, the Paul and Evanina Mackall Foundation Trust, Hope for Vision and the F.M. Kirby F
Investigative Ophthalmology & Visual Science April 2009, Vol.50, 3010. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      L. J. Stein, D. C. Chung, J. Bennicelli, Z. Wei, J. Bennett; Gene Targeting to Murine Retinal Bipolar Cells. Invest. Ophthalmol. Vis. Sci. 2009;50(13):3010.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Results: : Histological sections were evaluated for eGFP expression and colabeling of protein kinase C-2 (PKC-2), under fluorescence microscopy. Animals injected with AAV2/8 had extensive expression in the retinal bipolar cells, and moderate expression in retinal ganglion cells. AAV2/5 also resulted in expression in bipolar cells, but to a lesser degree than AAV2/8, but had additional expression in retinal pigment epithelial cells (RPE). AAV2/2 and 2/1 resulted in sparse expression in ganglion cells and RPE cells. Immunofluorescence revealed transduction of retinal bipolar cells in animals injected with AAV2/8 and 2/5. Delivery of the vector was well tolerated locally and systemically and animals showed no alteration in behavior or general well being.

Conclusions: : Adeno-associated virus 2/8 exhibits strong tropism to retinal bipolar cells and efficiently delivers transgenes to retinal bipolar cells. This vector could be useful in probing the biology of retinal bipolar cells and in strategies aimed at preventing or reversing blindness.

Keywords: bipolar cells • gene transfer/gene therapy • retinal degenerations: cell biology 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×