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A. K. Junk, B. Govindarajan, M. Algeciras, R. G. Salomon, B. Anand-Apte, S. K. Bhattacharya; Increased Isolevuglandin Modified Proteins in Glaucomatous Astrocytes. Invest. Ophthalmol. Vis. Sci. 2009;50(13):3623.
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Lipid oxidation has been proposed to be a factor in the pathophysiology of glaucoma. We investigated whether elevated levels of isolevuglandin (isoLGE2) protein adducts are associated with astrocytes derived from glaucomatous optic nerve head. In addition, how isoLGE2 protein adducts are altered following exposure of astrocytes to ambient and elevated pressure was examined.
Astrocytes were isolated from rat brain cortex and human optic nerve, and subjected to pressure treatments, Western blot analyses, liquid chromatography tandem mass spectrometry and immunocytochemistry.
Elevated levels of isolevuglandin (isoLGE2) protein adducts were associated with astrocytes derived from glaucomatous optic nerve head compared to those from controls. Astrocytes subjected to in vitro pressure treatment results in increased levels of isoLGE2 protein adducts. Pressure exposure and recovery period affects isoLGE2 protein modification and pyridoxamine incubation results in decreased isoLGE2 protein adduct immunoreactivity when applied immediately after pressure treatment.
These results suggest that the elevated isoLGE2 protein adduct immunoreactivity observed in glaucomatous astrocytes may be due to chronic and/or prolonged exposure to pressure.
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