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C. M. Cebulla, M. Ruggeri, T. G. Murray, E. Hernandez; Optical Coherence Tomography in a Murine Retinal Detachment Model. Invest. Ophthalmol. Vis. Sci. 2009;50(13):3798.
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© ARVO (1962-2015); The Authors (2016-present)
Spectral domain optical coherence tomography (SD-OCT) was used to image retinal detachments in vivo, in a murine model of retinal detachment.
IACUC approval was obtained. Subretinal injections of 3-4 microliters of healon were delivered to the right eye of six 10-20 week-old mice. Evaluation of the fundus with an operating microscope was performed to document the detachment and fundus photography was performed. In vivo, non-contact, ultra high resolution SD-OCT imaging was performed on day 0, day 2, day 6 and day 14 to evaluate the detachment. Eyes were enucleated for histologic analysis.
The retinal detachment was confirmed by microscopy in all mice. SD-OCT detected the retinal detachment in 5/6 cases. The retinal detachment was not detected in one eye since it was very peripheral. The retina was partially detached in 3 cases and totally detached in 3 cases. The extent of the retinal detachment was evaluated by measuring the height of the subretinal healon. The retinal layers, including the photoreceptor layer, were evaluated. SD-OCT imaging revealed relative blurring and thickening of the layers of the detached retina on day zero compared to non-detached retina. The maximal elevation of the retinal detachment was difficult to image with SD-OCT on day zero since the height was at the border of the detection limit. Two days post-injection, the retina was mostly re-attached and limited areas of retinal detachment remained. The retinal layers were more clearly defined. SD-OCT imaging was limited on day 6 and 14 by cataract formation. Histologic analysis demonstrated retinal detachments by H & E staining.
SD-OCT is a promising technology to follow retinal detachment formation and resolution in a murine model.
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