April 2009
Volume 50, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2009
NMR-Based Metabolomic Analysis of Cerebrospinal Fluid and Serum in Neuro-Ophthalmological and Neurological Diseases - A Diagnostic Tool?
Author Affiliations & Notes
  • A. J. Sinclair
    Academic Unit of Ophthalmology, School of Immunity, Infection and Inflammation,
    Endocrinology, School of Experimental Medicine,
    University of Birmingham, Birmingham, United Kingdom
  • M. R. Viant
    School of Biosciences,
    University of Birmingham, Birmingham, United Kingdom
  • G. R. Wallace
    Academic Unit of Ophthalmology, School of Immunity, Infection and Inflammation,
    University of Birmingham, Birmingham, United Kingdom
  • A. K. Ball
    Neurology, School of Experimental Medicine,
    University of Birmingham, Birmingham, United Kingdom
  • M. A. Burdon
    Academic Unit of Ophthalmology, School of Immunity, Infection and Inflammation,
    University of Birmingham, Birmingham, United Kingdom
  • E. A. Walker
    Endocrinology, School of Experimental Medicine,
    University of Birmingham, Birmingham, United Kingdom
  • P. M. Stewart
    Endocrinology, School of Experimental Medicine,
    University of Birmingham, Birmingham, United Kingdom
  • S. P. Young
    Rheumatology, School of Immunity, Infection and Inflammation,
    University of Birmingham, Birmingham, United Kingdom
  • S. Rauz
    Academic Unit of Ophthalmology, School of Immunity, Infection and Inflammation,
    University of Birmingham, Birmingham, United Kingdom
  • Footnotes
    Commercial Relationships  A.J. Sinclair, None; M.R. Viant, None; G.R. Wallace, None; A.K. Ball, None; M.A. Burdon, None; E.A. Walker, None; P.M. Stewart, None; S.P. Young, None; S. Rauz, None.
  • Footnotes
    Support  Alex Sinclair is a Medical Research Council Clinical Research Training Fellow (UK) and the Academic Unit of Ophthalmology is supported by the Birmingham Eye Foundation (Registered (UK) Charity 257549)
Investigative Ophthalmology & Visual Science April 2009, Vol.50, 4036. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      A. J. Sinclair, M. R. Viant, G. R. Wallace, A. K. Ball, M. A. Burdon, E. A. Walker, P. M. Stewart, S. P. Young, S. Rauz; NMR-Based Metabolomic Analysis of Cerebrospinal Fluid and Serum in Neuro-Ophthalmological and Neurological Diseases - A Diagnostic Tool?. Invest. Ophthalmol. Vis. Sci. 2009;50(13):4036.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: : Establishing biomarkers for conditions affecting the central nervous system have to date proven to be problematic. Processes such as gene transcription, and environmental factors, can affect the metabolic fingerprint of tissues. The recent innovation of metabolomic analysis of biofluids has the capacity to integrate these bio-influences into a single biomarker profile. We therefore sought to define whether metabolomic biomarker profiling of cerebrospinal fluid (CSF) and serum could potentially distinguish between idiopathic intracranial hypertension (IIH), multiple sclerosis (MS) and cerebrovascular disease (CVD) from patients with mixed neurological diseases.

Methods: : Spectra of CSF (n=87) and serum (n=72) were acquired using 1H NMR spectroscopy. Multivariate pattern recognition analysis was used to identify disease specific metabolite biomarker profiles. The metabolite profiles were then used to predict the diagnosis of a prospectively collected cohort of patients (n=25).

Results: : CSF metabolite profiles were able to predict diagnosis with a sensitivity and specificity of 80% for both IIH and for MS. The CVD serum metabolite profile was 75% sensitive and specific. On analysing the second prospective patient cohort, the established metabolite biomarker profiles generated from the first cohort showed moderate ability to segregate patients with IIH and MS (sensitivity: specificity of 63%:75% and 67%:75%, respectively).

Conclusions: : These findings suggest that NMR spectroscopic metabolic profiling of CSF and serum can identify differences between IIH, MS, CVD and mixed neurological diseases. Metabolomics may, therefore, have the potential to be developed into a clinically useful diagnostic tool. The identification of disease-unique metabolites may also impart information on disease pathology.

Keywords: metabolism • neuro-ophthalmology: diagnosis • clinical research methodology 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×