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N. Dong, W. Li, W. Qin, H. Lin, C. Li, W. Chen, Z. Liu; Abnormal Epithelial Differentiation and Tear Film Alteration in Pinguecula. Invest. Ophthalmol. Vis. Sci. 2009;50(13):4275.
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To investigate differentiation of conjunctival epithelium and tear film function in pingueculae.
Twelve patients (12 eyes), who underwent simple excision for pingueculae, were enrolled in this study. Immunostaining_K14, K19, K10, MUC5AC, Pax6, P63, Ki67, and K16_was performed on pinguecula epithelium and normal conjunctival epithelium. The Schirmer I test as well as the tear film break-up time (TFBUT) were evaluated just prior to and 1 month after surgery.
Abnormal epidermal differentiation of pinguecula tissue, as evidenced by a decline in or absence of Pax6 expression, was accompanied by a decline in or absence of K19 keratin and MUC5AC, and an increase of in K10 and K14 keratin. Furthermore, pinguecula epithelium was actively proliferating, as evidenced by positive expression of Ki67, P63, and K16 keratin. The Schirmer I test did not indicate any significant differences pre- and post-operatively. However, the TFBUT was significantly prolonged 1 month post surgery compared to pre-surgery.
Pinguecula is a condition of abnormal epithelial differentiation. It is characterized by squamous proliferation and metaplasia, resulting in instability of tear film with normal basic tear secretion.
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