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Y. Zhang, Y. Hiromasa, T. Iwamoto, A. H. Conrad, J. M. Tomich, G. W. Conrad; Direct Profiling of Crystallines Distribution in Cornea Tissue Sections by MALDI Imaging Mass Spectrometry. Invest. Ophthalmol. Vis. Sci. 2009;50(13):4523.
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© ARVO (1962-2015); The Authors (2016-present)
Past studies have established that the cornea, like the lens, abundantly expresses a few water-soluble enzymes/proteins, which are classified as ubiquitous -, β-, and γ-crystallins. This study has investigated the physical distribution patterns and characterized the crystallins in corneas using MALDI imaging mass spectrometry (MALDI-IMS).
Snap liquid nitrogen frozen White Leghorn chicken embryo corneas were sectioned with a cryostat and transferred to glass slides. Matrix solution was deposited on the tissue surface, dried and then analyzed directly by MALDI mass spectrometry (Bruker Ultraflex II MALDI TOF/TOF).
Profiles of crystallin distributions in corneal tissue sections were achieved. For chick E18 corneal sections, molecular ions observed at m/z 19725 and m/z 19946 correspond to A crystallin and B crystallin, respectively. Multiple degradation products of A crystallin were also observed at m/z 13757, m/z 12320, m/z 10154 and m/z 11273. Degradation products of B crystallin were observed at m/z 8074 and m/z 4930. Localization of these crystallins in the cornea is currently in progress.
MALDI-IMS offers an improved method for mapping the physical distribution patterns of crystallins in individual corneal sections and will give new insight into the developing chick cornea.
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