April 2009
Volume 50, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2009
Direct Profiling of Crystallines Distribution in Cornea Tissue Sections by MALDI Imaging Mass Spectrometry
Author Affiliations & Notes
  • Y. Zhang
    Biology,
    Kansas State University, Manhattan, Kansas
  • Y. Hiromasa
    Biochemistry,
    Kansas State University, Manhattan, Kansas
  • T. Iwamoto
    Biochemistry,
    Kansas State University, Manhattan, Kansas
  • A. H. Conrad
    Biology,
    Kansas State University, Manhattan, Kansas
  • J. M. Tomich
    Biochemistry,
    Kansas State University, Manhattan, Kansas
  • G. W. Conrad
    Biology,
    Kansas State University, Manhattan, Kansas
  • Footnotes
    Commercial Relationships  Y. Zhang, None; Y. Hiromasa, None; T. Iwamoto, None; A.H. Conrad, None; J.M. Tomich, None; G.W. Conrad, None.
  • Footnotes
    Support  National Institutes of Health Grant EY000952 to GWC. NSF Major Research Instrumentation Program (MRI) 0521587 to Kansas State University.
Investigative Ophthalmology & Visual Science April 2009, Vol.50, 4523. doi:
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    • Get Citation

      Y. Zhang, Y. Hiromasa, T. Iwamoto, A. H. Conrad, J. M. Tomich, G. W. Conrad; Direct Profiling of Crystallines Distribution in Cornea Tissue Sections by MALDI Imaging Mass Spectrometry. Invest. Ophthalmol. Vis. Sci. 2009;50(13):4523.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : Past studies have established that the cornea, like the lens, abundantly expresses a few water-soluble enzymes/proteins, which are classified as ubiquitous -, β-, and γ-crystallins. This study has investigated the physical distribution patterns and characterized the crystallins in corneas using MALDI imaging mass spectrometry (MALDI-IMS).

Methods: : Snap liquid nitrogen frozen White Leghorn chicken embryo corneas were sectioned with a cryostat and transferred to glass slides. Matrix solution was deposited on the tissue surface, dried and then analyzed directly by MALDI mass spectrometry (Bruker Ultraflex II MALDI TOF/TOF).

Results: : Profiles of crystallin distributions in corneal tissue sections were achieved. For chick E18 corneal sections, molecular ions observed at m/z 19725 and m/z 19946 correspond to A crystallin and B crystallin, respectively. Multiple degradation products of A crystallin were also observed at m/z 13757, m/z 12320, m/z 10154 and m/z 11273. Degradation products of B crystallin were observed at m/z 8074 and m/z 4930. Localization of these crystallins in the cornea is currently in progress.

Conclusions: : MALDI-IMS offers an improved method for mapping the physical distribution patterns of crystallins in individual corneal sections and will give new insight into the developing chick cornea.

Keywords: crystallins • cornea: stroma and keratocytes 
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