April 2009
Volume 50, Issue 13
ARVO Annual Meeting Abstract  |   April 2009
Glycinergic Contacts Between AII Amacrine Cells and Off Ganglion Cells in the Rabbit Retina
Author Affiliations & Notes
  • W.-L. Liu
    Ophthalmology, UT-Houston Medical School, Houston, Texas
  • S. C. Massey
    Ophthalmology, UT-Houston Medical School, Houston, Texas
  • Footnotes
    Commercial Relationships  W.-L. Liu, None; S.C. Massey, None.
  • Footnotes
    Support  NEI: EY06515, Core Grant EY 10708 and Research to Prevent Blindness: an unrestricted award to the Dept. of Ophthalmology and Visual Science, UT Medical School at Houston.
Investigative Ophthalmology & Visual Science April 2009, Vol.50, 4560. doi:
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    • Get Citation

      W.-L. Liu, S. C. Massey; Glycinergic Contacts Between AII Amacrine Cells and Off Ganglion Cells in the Rabbit Retina. Invest. Ophthalmol. Vis. Sci. 2009;50(13):4560.

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      © ARVO (1962-2015); The Authors (2016-present)

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Purpose: : Recently, it was proposed that OFF ganglion cells receive a direct inhibitory input from AII amacrine cells. This pathway provides a disinhibitory input at low contrast (Manookin et al., 2008). In addition, -like ganglion cells in the mouse retina express kinetically fast glycine receptors composed of 1 subunits (Majumdar et al., 2007). Here, we provide anatomical evidence in support of lobular AII contacts via glycine 1 receptors with OFF ganglion cells.

Methods: : OFF ganglion cells in wholemount rabbit retina were dye injected with Neurobiotin. After fixation, the AII amacrine cell population was labeled with an antibody against calretinin and glycine receptors were marked with an antibody against the 1 subunit. These materials were analyzed by triple-label confocal microscopy.

Results: : OFF ganglion cells showed the typical dendritic morphology. They were stratified in sublamina a and dye-coupled to other OFF ganglion cells and unidentified amacrine cells. The lobules of AII amacrine cells made close contacts at many points along the dendrites of individual OFF ganglion cells. At these potential synaptic sites, we also found punctate labeling for the glycine 1 subunit.

Keywords: ganglion cells • amacrine cells • retina 

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