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L. Contreras-Ruiz, U. Schulze, Y. Diebold, F. Paulsen; Trefoil Factor Family Peptide 3 (TFF3) Affects Tight Junction Proteins in Corneal Epithelial Cells. Invest. Ophthalmol. Vis. Sci. 2009;50(13):4611.
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The aim of the present study was to evaluate a potential TFF3-induced effect on the expression of tight junction proteins in corneal epithelial cells.
We first analyzed the expression of several proteins of the tight junction complex, both transmembrane proteins (occludin and claudins 1 and 2) and cytoplasmatic plaque proteins (the zonula occludens ZO-1 and -2) in human corneal epithelial cells (Araki-Sasaki-HCE cell line) by RT-PCR. Then, HCE cells were cultured on 35mm dishes and stimulated with recombinant human TFF3 (rhTFF3) 0.3mg/ml) for 6 and 24h. The expression of tight junction proteins was studied using real time RT-PCR. The comparative Ct method was used to analyze the results. Immunofluorescence studies of these proteins are currently ongoing.
ZO-1, ZO-2, claudin-1, claudin-2 and occludin were detected in HCE cells in basal conditions. After rhTFF3 treatment for 6h, real time RT-PCR revealed an increased mRNA expression level for claudin-1, claudin-2 and occludin, whereas rhTFF3 stimulation for 24h did not significantly changed the expression. In contrast, ZO-1 showed an upregulated expression, whereas ZO-2 expression was decreased after rhTFF3 treatment for 24h.
TFF3 might play a role in the regulation of the corneal barrier function by modulating tight junction protein expression.
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