April 2009
Volume 50, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2009
Trefoil Factor Family Peptide 3 (TFF3) Affects Tight Junction Proteins in Corneal Epithelial Cells
Author Affiliations & Notes
  • L. Contreras-Ruiz
    Ocular Surface Group, IOBA-University of Valladolid, Valladolid, Spain
    Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER BBN), Valladolid, Spain
  • U. Schulze
    Department of Anatomy and Cell Biology, Martin-Luther-University Halle-Wittenberg, Halle/Saale, Germany
  • Y. Diebold
    Ocular Surface Group, IOBA-University of Valladolid, Valladolid, Spain
    Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER BBN), Valladolid, Spain
  • F. Paulsen
    Department of Anatomy and Cell Biology, Martin-Luther-University Halle-Wittenberg, Halle/Saale, Germany
  • Footnotes
    Commercial Relationships  L. Contreras-Ruiz, None; U. Schulze, None; Y. Diebold, None; F. Paulsen, None.
  • Footnotes
    Support  FEDER-CICYT MAT2007-64626-C02-01/C02-02, and FPU Scholarship Program, Ministry of Education, Spain. DFG grant PA738/9-2; BMBF Roux program grants FKZ 9/18, 12/08, 13/08, Sicca Forschungsförde
Investigative Ophthalmology & Visual Science April 2009, Vol.50, 4611. doi:
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    • Get Citation

      L. Contreras-Ruiz, U. Schulze, Y. Diebold, F. Paulsen; Trefoil Factor Family Peptide 3 (TFF3) Affects Tight Junction Proteins in Corneal Epithelial Cells. Invest. Ophthalmol. Vis. Sci. 2009;50(13):4611.

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Abstract

Purpose: : The aim of the present study was to evaluate a potential TFF3-induced effect on the expression of tight junction proteins in corneal epithelial cells.

Methods: : We first analyzed the expression of several proteins of the tight junction complex, both transmembrane proteins (occludin and claudins 1 and 2) and cytoplasmatic plaque proteins (the zonula occludens ZO-1 and -2) in human corneal epithelial cells (Araki-Sasaki-HCE cell line) by RT-PCR. Then, HCE cells were cultured on 35mm dishes and stimulated with recombinant human TFF3 (rhTFF3) 0.3mg/ml) for 6 and 24h. The expression of tight junction proteins was studied using real time RT-PCR. The comparative Ct method was used to analyze the results. Immunofluorescence studies of these proteins are currently ongoing.

Results: : ZO-1, ZO-2, claudin-1, claudin-2 and occludin were detected in HCE cells in basal conditions. After rhTFF3 treatment for 6h, real time RT-PCR revealed an increased mRNA expression level for claudin-1, claudin-2 and occludin, whereas rhTFF3 stimulation for 24h did not significantly changed the expression. In contrast, ZO-1 showed an upregulated expression, whereas ZO-2 expression was decreased after rhTFF3 treatment for 24h.

Conclusions: : TFF3 might play a role in the regulation of the corneal barrier function by modulating tight junction protein expression.

Keywords: cornea: epithelium • inflammation • cornea: basic science 
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