Purchase this article with an account.
Y.-H. Liu, F. Zhuang; Eaf2 Facilitates the Breakdown of Nucleolus. Invest. Ophthalmol. Vis. Sci. 2009;50(13):4631.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
The purpose of the current study is to reveal cellular function of Eaf2 in influencing structural integrity of the nucleolus in response to UV damage.
GFP tagged Eaf2 was introduced into various cell types by liposome-mediated transfection. Cells were irradiated under UV light. GFP and immunofluorescence to nucleolar proteins were imaged using confocal microscopy.
Our analysis showed an intriguing distribution pattern of Eaf2-GFP. Under normal culturing conditions, Eaf2-GFP rapidly moves into the cell nucleus and degrades. When transfected cells are exposed to UV irradiation, Eaf2-GFP relocalizes into the nucleolus. This nucleolar relocalization phenomena can be induced in the presence of Ing1b without UV irradiation. Co-expression of Ing1b and Eaf2 resulted in the disappearance of the nucleolar structure in a step-wise manner.
Eaf2 may be a critical molecular player in mediating or facilitating nucleolar breakdown in response to genotoxic stress or during mitosis.
This PDF is available to Subscribers Only