April 2009
Volume 50, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2009
Eaf2 Facilitates the Breakdown of Nucleolus
Author Affiliations & Notes
  • Y.-H. Liu
    Ophthalmology, Univ of Southern California, Los Angeles, California
  • F. Zhuang
    Ophthalmology, Univ of Southern California, Los Angeles, California
  • Footnotes
    Commercial Relationships  Y.-H. Liu, None; F. Zhuang, None.
  • Footnotes
    Support  NIH grant EY015417
Investigative Ophthalmology & Visual Science April 2009, Vol.50, 4631. doi:
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      Y.-H. Liu, F. Zhuang; Eaf2 Facilitates the Breakdown of Nucleolus. Invest. Ophthalmol. Vis. Sci. 2009;50(13):4631.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : The purpose of the current study is to reveal cellular function of Eaf2 in influencing structural integrity of the nucleolus in response to UV damage.

Methods: : GFP tagged Eaf2 was introduced into various cell types by liposome-mediated transfection. Cells were irradiated under UV light. GFP and immunofluorescence to nucleolar proteins were imaged using confocal microscopy.

Results: : Our analysis showed an intriguing distribution pattern of Eaf2-GFP. Under normal culturing conditions, Eaf2-GFP rapidly moves into the cell nucleus and degrades. When transfected cells are exposed to UV irradiation, Eaf2-GFP relocalizes into the nucleolus. This nucleolar relocalization phenomena can be induced in the presence of Ing1b without UV irradiation. Co-expression of Ing1b and Eaf2 resulted in the disappearance of the nucleolar structure in a step-wise manner.

Conclusions: : Eaf2 may be a critical molecular player in mediating or facilitating nucleolar breakdown in response to genotoxic stress or during mitosis.

Keywords: radiation damage: light/UV • cornea: basic science • development 
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