Purchase this article with an account.
T. Hongyok, M. Hornof, J. M. Chae, Y. J. Shin, D. Na, L. Li, R. S. Chuck; Thiolated Chitosan for the Treatment of Dry Eye - Evaluation in the Botulinum Toxin-B Induced Dry Eye Mouse Model. Invest. Ophthalmol. Vis. Sci. 2009;50(13):4654.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
The role of a topically applied thiolated polymer, chitosan-N-acetylcysteine conjugate (C-NAC), was investigated in a dry eye mouse model. It is postulated that interaction between thiol groups of the C-NAC with cysteine-rich mucins (MUC5AC) increases tear film stability.
Dry eye was induced by injecting botulinum toxin B (BTX-B) in the right eye lacrimal glands of CBA/J mice. Eye drops containing C-NAC 0.5%, C-NAC 0.3%, vehicle (control), artificial tear (Artelac® EDO®) or fluorometholone (FML®) were applied in a masked fashion to group A-E, respectively, twice per day starting from day 3 until 4 weeks after BTX-B injection. Corneal fluorescein staining was periodically recorded. Quantitative Real-Time RT-PCR and immunofluorescence staining were performed at the end of the study to evaluate expression of inflammatory cytokines in ocular surface tissues.
Mice treated with C-NAC 0.5% and fluorometholone showed a downward trend in corneal staining compared to the other 3 groups, which was not statistically significant. C-NAC formulations, fluorometholone and artificial tear significantly decreased IL-1β, IL-10, IL-12, and TNF- expression in ocular surface tissues in comparison to the control (Tab. 1).
This study has demonstrated the potential usefulness of the BTX-B-induced dry eye mouse model to evaluate new dry eye treatment strategies. Evaluation of important molecular biomarkers of dry eye disease suggests that C-NAC may have some protective ocular surface properties. However, clinical data did not indicate statistically significant improvement of tear film stability in any of the groups tested.
This PDF is available to Subscribers Only