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N. Lara, S. Nakao, K. Noda, M. Ahmed, S. Frimmel, Y. Ito, M. I. Melhorn, Z. Ablonczy, A. Hafezi-Moghadam; Atrial Natriuretic Peptide (ANP) Blocks VEGF-Induced Blood-Retina-Barrier (BRB) Leakage. Invest. Ophthalmol. Vis. Sci. 2009;50(13):4990.
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ANP, a cardiovascular cyclic peptide, blocks VEGF signaling and production in vitro. However, the role of ANP in the eye is not understood. This study investigates the contribution of ANP to VEGF-induced BRB leakage in vivo.
Brown Norway rats were injected intravitreally with 5µL of ANP (0.1µM), 5µL ANP and VEGF (10ng/ml), or 5µL vehicle (PBS). BRB breakdown was quantified using the Evans Blue dye technique, 24 hrs after intravitreal injections. To determine the effect of ANP on barrier function, trans-epithelial electrical resistance (TEER) measurements were performed in human retinal vascular endothelial cells. The cells were treated with VEGF (100ng/mL) or vehicle in the presence or absence of ANP (1µM). TEER was measured 0.5, 1, and 3 hrs after ANP administration.
In vivo, concomitant intravitreous injection of VEGF and ANP significantly reduced VEGF-induced BRB breakdown (108.5±7.7 mL/g/h) vs VEGF-treated controls (406.6±68.7 mL/g/h, n=9, p<0.001). In vitro, the VEGF-induced reduction of TEER in cultured endothelial cells (90.8±2.3 %) was significantly inhibited in the presence of ANP (99.6 90.8±2.6%, n=6, p<0.05).
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