April 2009
Volume 50, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2009
Atrial Natriuretic Peptide (ANP) Blocks VEGF-Induced Blood-Retina-Barrier (BRB) Leakage
Author Affiliations & Notes
  • N. Lara
    Angiogenesis Laboratory, MEEI-Harvard Medical School, Cambridge, Massachusetts
  • S. Nakao
    Angiogenesis Laboratory, MEEI-Harvard Medical School, Cambridge, Massachusetts
  • K. Noda
    Angiogenesis Laboratory, MEEI-Harvard Medical School, Cambridge, Massachusetts
  • M. Ahmed
    Angiogenesis Laboratory, MEEI-Harvard Medical School, Cambridge, Massachusetts
  • S. Frimmel
    Angiogenesis Laboratory, MEEI-Harvard Medical School, Cambridge, Massachusetts
  • Y. Ito
    Angiogenesis Laboratory, MEEI-Harvard Medical School, Cambridge, Massachusetts
  • M. I. Melhorn
    Angiogenesis Laboratory, MEEI-Harvard Medical School, Cambridge, Massachusetts
  • Z. Ablonczy
    Department of Ophthalmology, Medical University of South Carolina, Charleston, South Carolina
  • A. Hafezi-Moghadam
    Angiogenesis Laboratory, MEEI-Harvard Medical School, Cambridge, Massachusetts
  • Footnotes
    Commercial Relationships  N. Lara, None; S. Nakao, None; K. Noda, None; M. Ahmed, None; S. Frimmel, None; Y. Ito, None; M.I. Melhorn, None; Z. Ablonczy, None; A. Hafezi-Moghadam, None.
  • Footnotes
    Support  NIH EY014793-01, HL086933 and AI050775, Massachusetts Lions Eye Research Fund Inc., Marion W. and Edward F. Knight AMD Fund, and Research to Prevent Blindness.
Investigative Ophthalmology & Visual Science April 2009, Vol.50, 4990. doi:
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      N. Lara, S. Nakao, K. Noda, M. Ahmed, S. Frimmel, Y. Ito, M. I. Melhorn, Z. Ablonczy, A. Hafezi-Moghadam; Atrial Natriuretic Peptide (ANP) Blocks VEGF-Induced Blood-Retina-Barrier (BRB) Leakage. Invest. Ophthalmol. Vis. Sci. 2009;50(13):4990.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : ANP, a cardiovascular cyclic peptide, blocks VEGF signaling and production in vitro. However, the role of ANP in the eye is not understood. This study investigates the contribution of ANP to VEGF-induced BRB leakage in vivo.

Methods: : Brown Norway rats were injected intravitreally with 5µL of ANP (0.1µM), 5µL ANP and VEGF (10ng/ml), or 5µL vehicle (PBS). BRB breakdown was quantified using the Evans Blue dye technique, 24 hrs after intravitreal injections. To determine the effect of ANP on barrier function, trans-epithelial electrical resistance (TEER) measurements were performed in human retinal vascular endothelial cells. The cells were treated with VEGF (100ng/mL) or vehicle in the presence or absence of ANP (1µM). TEER was measured 0.5, 1, and 3 hrs after ANP administration.

Results: : In vivo, concomitant intravitreous injection of VEGF and ANP significantly reduced VEGF-induced BRB breakdown (108.5±7.7 mL/g/h) vs VEGF-treated controls (406.6±68.7 mL/g/h, n=9, p<0.001). In vitro, the VEGF-induced reduction of TEER in cultured endothelial cells (90.8±2.3 %) was significantly inhibited in the presence of ANP (99.6 90.8±2.6%, n=6, p<0.05).

Keywords: vascular endothelial growth factor 
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