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R. Somani, S. R. Russell, J. C. Folk, H. C. Boldt, K. M. Gehrs, R. F. Mullins, V. B. Mahajan; Immunohistochemical Study of Diamond-Dusted Tano Scratchers After Macular Surgery. Invest. Ophthalmol. Vis. Sci. 2009;50(13):4457.
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To identify the tissues removed following application of Tano diamond-dusted scrapers to the macula.
A Tano diamond-dusted scraper was applied to the macula of human donor eyes. The retina was fixed in 4% paraformaldehyde and processed for immunohistochemistry. Anti-laminin and DAPI stain were used to identify the internal limiting membrane and cell nuclei. A similar analysis was performed directly on the instrument tips following vitrectomy for macular hole.
The macula showed distinct areas of limited and complete ILM tissue disruption. The tips of the Tano diamond-dusted tip scraper showed laminin positive tissue fragments and limited numbers of cells.
When applied to the macula, brushing the Tano diamond-dusted scraper disrupts the ILM of the retina and may remove surface cells. This technique may be sufficient to achieve macular hole closure at rates comparable to complete ILM peeling.
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