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P. J. McDonnell, III, S. Martins, J. Castro-Combs, W. Camacho, P. Whittman, J. L. Hicks, A. M. De Marzo, C. G. Eberhart, A. Behrens; Dermcidin Expression in the Eye - Potential Protective Mechanism Against Ocular Bacterial Invasion. Invest. Ophthalmol. Vis. Sci. 2009;50(13):5110.
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Dermcidin (DCD), a recently discovered antimicrobial peptide, has been previously detected in the aqueous humor of New Zealand white rabbits. The purpose of this study is to detect the presence of DCD in human intraocular tissues as a potential mechanism of innate defense against intraocular infections.
We investigated the immunoexpression of this novel and established antimicrobial peptide in the human eye using a mouse monoclonal antibody raised against human DCD chromatographically purified from human sweat. 5-µm vertical paraffin sections were prepared and immediately processed. The sections were incubated for 30 minutes with a 1:600 dilution of mouse monoclonal IgM raised against human DCD. Regional production of this peptide was detected in several intraocular structures.
DCD was constitutively expressed in human sweat glands of all skin biopsies. Immunoreactivity was also observed in human corneal epithelium, endothelium and ciliary body. Human corneal endothelium and ciliary body seem to express DCD gene constitutively.
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