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L. Cui, Y. Guan, Y. Wu, J.-Y. Xu, W. Li, G.-T. Xu; Potential of Neural Stem Cells in Retinal Degeneration Therapy. Invest. Ophthalmol. Vis. Sci. 2009;50(13):5135.
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© ARVO (1962-2015); The Authors (2016-present)
By subretinal injection of fetal derived and mES differentiated neural progenitors cells (NPCs) in mice suffered from retinal degeneration to explore their potential in the cell therapy application.
Primary cultured NPCs were separated from postnatal 1 day GFP transgene mouse retina. D3-ES derived NPCs were obtained after the neural determination culture, and fluorescence activated cell sorting (FACS)-based lineage selection by Hes1-driven enhanced green fluorescent protein (EGFP) expression. The fetal and mES differentiated NPCs were then transplanted, as donor cells, into the subretinal space of Sodium Iodate (SI) treated mice. The therapeutic effects were evaluated by direct fundus observation and electroretinograph (ERG) between 2 and 4 weeks following the cell transplantation. The transplantation efficiency of donor cells was also examined for their survival, integration, and differentiation into retinal cells after the subretinal transplantation at the same intervals.
The present study reported an efficient procedure for the generation of NPCs from mES cells in vitro. It combined the embryoid body (EB) culture and addition of extracellular inductive signals. The induction efficiency was confirmed by quantitative PCR and immunocytochemistry. Three weeks after the fetal and mES derived NPCs transplantation, accompanying the improvement of the eye examinations, EGFP-expressing cells found scattered in the retina and adopted retina-like morphologies. Although, transplantation experiments revealed localization of donor cells near the injection site, there were some EGFP expressing cells migrate into different layers of the retina, like inner nuclear layer (INL), ganglion cell layer (GCL) and outer nuclear layer (ONL).
The present work has indicated NPCs could be good donor cells for rescue on the degenerative retinal cells.
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