April 2009
Volume 50, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2009
3D Culture of Postnatal Retinal Stem Cells Using Self Assembling Polymers
Author Affiliations & Notes
  • Y. Arsenijevic
    Unit of Gene Therapy & Stem Cell Biology, Jules-Gonin Eye Hospital, University of Lausanne, Switzerland
  • M. Eberhardt
    Unit of Gene Therapy & Stem Cell Biology, Jules-Gonin Eye Hospital, University of Lausanne, Switzerland
  • T. P. Kraehenbuehl
    Institute of Bioengineering, Federal Institute of Technology Lausanne, Switzerland
  • M. Tekaya
    Unit of Gene Therapy & Stem Cell Biology, Jules-Gonin Eye Hospital, University of Lausanne, Switzerland
  • M. Lutolf
    Institute of Bioengineering, Federal Institute of Technology Lausanne, Switzerland
  • J. A. Hubbell
    Institute of Bioengineering, Federal Institute of Technology Lausanne, Switzerland
  • Footnotes
    Commercial Relationships  Y. Arsenijevic, None; M. Eberhardt, None; T.P. Kraehenbuehl, None; M. Tekaya, None; M. Lutolf, None; J.A. Hubbell, None.
  • Footnotes
    Support  Velux foundation
Investigative Ophthalmology & Visual Science April 2009, Vol.50, 5152. doi:
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      Y. Arsenijevic, M. Eberhardt, T. P. Kraehenbuehl, M. Tekaya, M. Lutolf, J. A. Hubbell; 3D Culture of Postnatal Retinal Stem Cells Using Self Assembling Polymers. Invest. Ophthalmol. Vis. Sci. 2009;50(13):5152.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : We have previously shown that retinal stem cells (RSCs) can be isolated from the radial glia population of the newborn mouse retina (Angénieux et al., 2006). These RSCs have a great capacity to renew and to generate a large number of neurons including cells differentiated towards the photoreceptor lineage (Mehri-Soussi et al., 2006). However, recent published results from our lab revealed that such cells have a poor integration and survival rate after grafting. The uncontrolled environment of a retina seems to prevent good integration and survival after grafting in vivo. To bypass this problem, we are evaluating the possibility of generating in vitro a hemi-retinal tissue before transplantation.

Methods: : RSC were expanded and cells passaged <10 were seeded in a solution containing poly-ethylene-glycol (PEG) polymer based hydrogels crosslinked with peptides that are chosen to be substrates for matrix metalloproteinases. Various doses of cross linkers peptides allowing connections between PEG polymers were tested. Different growth factors were studied to stimulate cell proliferation and differentiation.

Results: : Cells survived only in the presence of EGF and FGF-2 and generated colonies with a sphere shape. No cells migrated within the gel. To improve the migration and the repartition of the cells in the gels, the integrin ligand RGDSP was added into the gel. In the presence of FGF-2 and EGF, newly formed cell clusters appeared by cell proliferation within several days, but again no outspreading of cells was observed. No difference was even seen when the stiffness of the hydrogels or the concentration of the integrin ligand RGDSP were changed. However, our preliminary results show that RSCs still form spheres when laminin is entrapped in the gel, but they started to spread out having a neuronal morphology after around 2 weeks. The neuronal population was assessed by the presence of the neuronal marker b-tubulin-III. This differentiation was achieved after successive steps of stimulations including FGF-2 and EGF, and then only FGF-2. Glial cells were also present. Further characterizations are under process.

Conclusions: : RSC can be grown in 3D. Preliminary results show that neuronal cell phenotype acquisition can be instructed by exogenous stimulations and factors linked to the gel. Further developments are necessary to form a homogenous tissue containing retinal cells.

Keywords: retinal culture • regeneration • transplantation 
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