April 2009
Volume 50, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2009
Imipramine Against Lipopolysaccharide-Induced Apoptosis in Retinal Stem Cells Mediated by Activation of BDNF Pathway
Author Affiliations & Notes
  • C.-H. Peng
    Ophthalmology, Shin Kong Wu Ho-Su Memorial Hospital, Taipei, Taiwan
  • S.-H. Chiou
    Medical Research and Education, Taipei Veterans General Hospital, Taipei, Taiwan
  • C.-K. Cheng
    Ophthalmology, Shin Kong Wu Ho-Su Memorial Hospital, Taipei, Taiwan
  • Footnotes
    Commercial Relationships  C.-H. Peng, None; S.-H. Chiou, None; C.-K. Cheng, None.
  • Footnotes
    Support  SKH-8302-97-DR-25
Investigative Ophthalmology & Visual Science April 2009, Vol.50, 5154. doi:
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      C.-H. Peng, S.-H. Chiou, C.-K. Cheng; Imipramine Against Lipopolysaccharide-Induced Apoptosis in Retinal Stem Cells Mediated by Activation of BDNF Pathway. Invest. Ophthalmol. Vis. Sci. 2009;50(13):5154.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : Depression is accompanied by the activation of the inflammatory-response system, and increased production of proinflammatory cytokines may play a role in the pathophysiology of depressive disorders. Imipramine (IM), a tricyclic antidepressant drug, has recently been shown to promote neurogenesis. However, whether IM elicits a neuroprotective or anti-inflammatory effect, or promotes the differentiation of retinal stem cells (RSCs) remains to be elucidated.

Methods: : We cultured RSCs derived from the adult rats as an in vitro model to evaluate the RSCs drug-modulation effects of IM.

Results: : Our results showed that IM treatment significantly increased the proliferation rate of RSCs, and up-regulated the mRNA and protein expression of BDNF in Day-7 IM-treated RSCs. Similar to BDNF-treated effect, incubation of RSCs with IM increased Bcl-2 protein levels and further prevented lipopolysaccharide (LPS)-induced apoptosis. Inhibition of BDNF expression with small interfering RNA (siRNA) significantly decreased Bcl-2 protein levels and abrogated the neuroprotective effects of IM against LPS-induced apoptosis in IM-treated RSCs. Moreover, using microdialysis with high performance liquid chromatography -electrochemical detection (HPLC-ED), the functional release of serotonin in the process of serotoninergic differentiation of IM-treated RSCs was concomitantly increasing and was mediated by the activation of the BDNF pathway/Bcl-2 cascades.

Keywords: regeneration • signal transduction: pharmacology/physiology • retina 
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