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Y. Okunuki, Y. Usui, N. Katai, M. Takeuchi, T. Kezuka, Y. Wakabayashi, H. Goto; Analysis of Multiple Chemokines and Growth Factors in the Vitreous of Patients With Branch Retinal Vein Occlusion. Invest. Ophthalmol. Vis. Sci. 2009;50(13):5397.
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© ARVO (1962-2015); The Authors (2016-present)
To analyze intravitreous concentrations of multiple chemokines and growth factors in patients with branch retinal vein occlusion (BRVO) and to compare the results with those of diabetic retinopathy (DR).
Eighteen BRVO (age 67.8±6.2, 9 men and 9 female), 14 DR (age 65.9±3.5, 8 men and 6 female), and 15 control subjects (age 65.3±8.7, 7 men and 8 female) were enrolled in this study. Among BRVO and DR patients, those with macular edema but no vitreous hemorrhage and new vessel were selected. Patients with a history of previous ocular surgery were excluded. Control subjects consisted of 10 patients with macular hole and 5 with epiretinal membrane. Vitreous and blood samples were obtained at the time of vitrectomy. Vitreous and serum concentrations of VEGF, IL-8, MCP-1, Mig, IP-10, basic FGF, GM-CSF, and RANTES were quantified using a FACS Caliber flow cytometer.
VEGF, IL-8, MCP-1, Mig, IP-10 were detected in the vitreous of BRVO patients and the concentrations were 160.0, 46.9, 1596.0, 90.1, and 155.2 pg/ml, respectively. IL-8 concentration in the vitreous was significantly higher in BRVO patients compared with DR patients (24.5 pg/ml) (p<0.05). In BRVO patients, vitreous concentration of IL-8 correlated significantly with vitreous concentrations of VEGF (p<0.0001), MCP-1 (p<0.0001), Mig (p<0.05), and IP-10 (p<0.01). All the factors detectable in the vitreous were higher in BRVO patients compared with controls. In BRVO patients, vitreous concentrations of VEGF and MCP-1 were higher than serum concentrations, but vitreous concentrations of IL-8, Mig, and IP-10 were not significantly different from serum concentrations.
Intravitreous VEGF, MCP-1, Mig, and IP-10 and especially IL-8 may contribute to the pathogenesis of BRVO.
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