April 2009
Volume 50, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2009
Metabolic Profile of Porcine Corneas after Photodynamic Cross-linking Treatment
Author Affiliations & Notes
  • T. Kryczka
    Institute of Neuroscience, NTNU, Trondheim, Norway
  • G. Wollensak
    Dep. of Ophthalmology, Eye Laser Institute, Martin Luther University, Halle, Germany
  • S. Sel
    Dep. of Ophthalmology, Eye Laser Institute, Martin Luther University, Halle, Germany
  • A. Midelfart
    Institute of Neuroscience, NTNU, Trondheim, Norway
    Dep. of Ophthalmology, University Hospital, Trondheim, Norway
  • Footnotes
    Commercial Relationships  T. Kryczka, None; G. Wollensak, None; S. Sel, None; A. Midelfart, None.
  • Footnotes
    Support  Norwegian Research Council Grant No: 170768
Investigative Ophthalmology & Visual Science April 2009, Vol.50, 5487. doi:
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      T. Kryczka, G. Wollensak, S. Sel, A. Midelfart; Metabolic Profile of Porcine Corneas after Photodynamic Cross-linking Treatment. Invest. Ophthalmol. Vis. Sci. 2009;50(13):5487.

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Abstract

Purpose: : Keratoconus is a noninflammatory conelike ectasia of the cornea, which progresses over time leading to substantial reduction of vision and discomfort. Photodynamic collagen cross-linking using ultraviolet A (UVA) irradiation and the photosensitizer riboflavin has been recently introduced as a new possible treatment of this disease. The aim of this study is to examine the influence of the cross-linking treatment on metabolic profile of corneas.

Methods: : 36 corneal buttons with 10 mm diameter were trephined from fresh post mortem porcine eyes; the epithelium was removed completely in all samples. 12 porcine corneas were used as controls. 12 corneas were cross-linked with the photosensitizer riboflavin (1 mg riboflavin-5-phosphate in 10 ml 20% dextran T-500) and UVA irradiation for 30 minutes using a double UVA diode (370 nm, 3 mW/cm2). Another 12 were obtained after the same cross-linking procedure, just omitting dextran in the photosensitizer solution. After treatment, the samples were immediately frozen at -80 ºC. The metabolic profiles of the samples were investigated with HR MAS (High Resolution Magic Angle Spinning) 1H NMR (Nuclear Magnetic Resonance) spectroscopy (14.1 T) operating at 600.132 MHz.

Results: : 20 metabolites were assigned in HR MAS 1H NMR spectra. Corneas exposed to cross-linking with riboflavin/dextran solution and UVA displayed significant differences in levels of leucine, glutathione and formate in comparison to the controls. Corneas cross-linked with riboflavin and UVA showed significant differences in levels of alanin, arginine, lactate and tryptophan in comparison to the controls.

Conclusions: : The study presents the metabolic profiles of porcine corneas after photodynamic collagen cross-linking treatment. This treatment option seems to be safe as the levels of most of the analysed water soluble metabolites were not significantly changed after this procedure and the cross-linking process did not seem to induce major alterations in the collagen amino acid composition. Then the questions arise about the long-term effect of photodynamic collagen cross-linking on metabolic profile of corneas and the nature of the collagen cross-links. Further research could explore the above issues.

Keywords: keratoconus • photodynamic therapy • cornea: basic science 
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