April 2009
Volume 50, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2009
Spontaneous Meibomianitis in AIRE-Deficient Mice
Author Affiliations & Notes
  • C. S. Hwang
    Ophthalmology, Ocular Surface Center, Cullen Eye Institute, BCM, Houston, Texas
  • S. Yeh
    Ophthalmology, Ocular Surface Center, Cullen Eye Institute, BCM, Houston, Texas
  • C. S. De Paiva
    Ophthalmology, Ocular Surface Center, Cullen Eye Institute, BCM, Houston, Texas
  • K. Trinca
    Ophthalmology, Ocular Surface Center, Cullen Eye Institute, BCM, Houston, Texas
  • A. Lingappan
    Ophthalmology, Ocular Surface Center, Cullen Eye Institute, BCM, Houston, Texas
  • J. K. Rafati
    Ophthalmology, Ocular Surface Center, Cullen Eye Institute, BCM, Houston, Texas
  • W. J. Farley
    Ophthalmology, Ocular Surface Center, Cullen Eye Institute, BCM, Houston, Texas
  • D.-Q. Li
    Ophthalmology, Ocular Surface Center, Cullen Eye Institute, BCM, Houston, Texas
  • S. C. Pflugfelder
    Ophthalmology, Ocular Surface Center, Cullen Eye Institute, BCM, Houston, Texas
  • Footnotes
    Commercial Relationships  C.S. Hwang, None; S. Yeh, None; C.S. De Paiva, None; K. Trinca, None; A. Lingappan, None; J.K. Rafati, None; W.J. Farley, None; D.-Q. Li, None; S.C. Pflugfelder, None.
  • Footnotes
    Support  NIH Grant EY 11915, Research to Prevent Blindness, The Oshman Foundation, The William Stamps Farish Fund, Allergan, Inc., and the Milton Boniuk Resident Research Fund
Investigative Ophthalmology & Visual Science April 2009, Vol.50, 5517. doi:
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    • Get Citation

      C. S. Hwang, S. Yeh, C. S. De Paiva, K. Trinca, A. Lingappan, J. K. Rafati, W. J. Farley, D.-Q. Li, S. C. Pflugfelder; Spontaneous Meibomianitis in AIRE-Deficient Mice. Invest. Ophthalmol. Vis. Sci. 2009;50(13):5517.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : Patients with autoimmune polyendocrinopathy (APECED), a condition that results from a mutation in the Autoimmune Regulatory (Aire) gene, develop chronic ocular surface inflammatory disease, including meibomianitis. The purpose of this study is to examine the meibomian gland immunopathology in Aire-knockout and wild type mice.

Methods: : Eyes with corresponding lids from Aire-deficient and wild type (C57BL6/J) mice were surgically removed, frozen, sectioned, and stained for immune/inflammatory cell markers (CD4+, CD8+, CD11b+, CD45+) by immunohistochemistry. The densities of these cells were measured in the meibomian glands of these tissues.

Results: : There was a greater density of CD4+, CD11b+, and CD45+ in the meibomian glands of Aire-deficient mice compared to the wild-type mice (p < 0.01, p < 0.01, p < 0.001 respectively). In contrast, the number of CD8+ cells was significantly higher in the meibomian glands of wild type mice compared to Aire-deficient mice (p < 0.001).

Conclusions: : The meibomian glands in Aire-deficient mice have a more immunologically active environment compared to wild type mice. Defective immunoregulation in the Aire glands may be related to the lower density of CD8+ cells. The altered immune environment may be the basis for the chronic meibomianitis and ocular surface disease observed in APECED patients.

Keywords: inflammation • autoimmune disease 
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