April 2009
Volume 50, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2009
Real-time mapping of the corneal Sub-basal Nerve Plexus by in vivo Laser Scanning Confocal Microscopy
Author Affiliations & Notes
  • R. F. Guthoff
    Ophthalmology, University of Rostock, Rostock, Germany
  • A. Zhivov
    Ophthalmology, University of Rostock, Rostock, Germany
  • O. Stachs
    Ophthalmology, University of Rostock, Rostock, Germany
  • Footnotes
    Commercial Relationships  R.F. Guthoff, None; A. Zhivov, None; O. Stachs, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science April 2009, Vol.50, 5669. doi:
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    • Get Citation

      R. F. Guthoff, A. Zhivov, O. Stachs; Real-time mapping of the corneal Sub-basal Nerve Plexus by in vivo Laser Scanning Confocal Microscopy. Invest. Ophthalmol. Vis. Sci. 2009;50(13):5669.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Methods: : In vivo laser scanning confocal microscopy (HRTII - RCM) of the sub-basal nerve plexus was performed on normal eyes of healthy human subjects. The source data with a frame rate of 30Hz was used to create large-scale maps of the scanned area by means of the ART (automatic real time) composite mode. The algorithm attempts to align every single live image onto the so far mapped composite image by means of landmark feature based image processing. As a result of this spatial image transformation the same corneal structure is at the same pixel location in the transformed live image and in the composite image. Initially a single live image is used as the first instance of the composite image. The transformed live image is added to the composite image and a pixel by pixel averaging is being performed in the overlap area to reduce noise in the final image. Live images are rejected if their transformation parameters cannot be accurately determined. The final composite image is a large-field mosaic image of a variable size up to 2mm x 2mm.

Results: : Real-time mapping of the sub-basal nerve plexus was performed in large-scale up to a size of 2mm x 2mm on healthy subjects. The mapping quality is depending on the subject’s compliance and the examiner’s experience.

Conclusions: : The presented method enables a real-time in vivo mapping of the sub-basal nerve plexus which is stringently necessary for statistically firmed conclusions about morphometric plexus alterations. The developed method can be used for large-scale mapping of several two-dimensional structures which are imaged by using the HRTII - RCM laser scanning system.

Keywords: microscopy: confocal/tunneling • cornea: basic science • anatomy 
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